製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to MOX1
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide within Human MOX1 aa 50-150 conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P50221

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human Fetal Heart tissue lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab125712 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 27 kDa).

ターゲット情報

  • 機能
    Mesodermal transcription factor that plays a key role in somitogenesis and is specifically required for sclerotome development. Required for maintenance of the sclerotome polarity and formation of the cranio-cervical joints (PubMed:23290072, PubMed:24073994). Binds specifically to the promoter of target genes and regulates their expression. Activates expression of NKX3-2 in the sclerotome. Activates expression of CDKN1A and CDKN2A in endothelial cells, acting as a regulator of vascular cell proliferation. While it activates CDKN1A in a DNA-dependent manner, it activates CDKN2A in a DNA-independent manner. Required for hematopoietic stem cell (HSCs) induction via its role in somitogenesis: specification of HSCs occurs via the deployment of a specific endothelial precursor population, which arises within a sub-compartment of the somite named endotome.
  • 関連疾患
    Klippel-Feil syndrome 2, autosomal recessive
  • 配列類似性
    Contains 1 homeobox DNA-binding domain.
  • 細胞内局在
    Nucleus. Cytoplasm. Localizes predominantly in the nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Homeobox protein MOX-1 antibody
    • MEOX1 antibody
    • MEOX1_HUMAN antibody
    • Mesenchyme homeo box 1 antibody
    • Mesenchyme homeobox 1 antibody
    • MOX 1 antibody
    • MOX1 antibody
    see all

画像

  • Anti-MOX1 antibody (ab125712) at 1 µg/ml + Heart (Human) Whole Cell Lysate - fetal normal tissue at 25 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 27 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 65 kDa (possible non-specific binding),75 kDa (possible non-specific binding).

    Exposure time : 20 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab125712 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406

  • All lanes : Anti-MOX1 antibody (ab125712) at 1 µg/ml

    Lane 1 : Heart (Human) Whole Cell Lysate - fetal normal tissue
    Lane 2 : Heart (Human) Whole Cell Lysate - fetal normal tissue with Immunising peptide at 1 µg/ml

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 27 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa (possible non-specific binding),65 kDa (possible non-specific binding).

    Exposure time : 20 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab125712 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406

プロトコール

参考文献

ab125712 has not yet been referenced specifically in any publications.

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