製品の概要

  • 製品名Mouse VCAM1 ELISA Kit
    VCAM1 キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 1.5 pg/ml
  • 検出範囲
    2.05 pg/ml - 500 pg/ml
  • 添加回収試験

    96 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 97.36 84% - 107%
    Serum 96.43 82% - 105%
    Plasma 94.64 81% - 104%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Mouse
  • 製品の概要

    Abcam’s VCAM1 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse VCAM1 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Mouse VCAM1 coated on a 96-well plate. Standards and samples are pipetted into the wells and VCAM1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse VCAM1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VCAM1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
  • 組織特異性Expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.
  • 配列類似性Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
  • ドメインEither the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion.
  • 翻訳後修飾Sialoglycoprotein.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 別名
    • CD106
    • CD106 Antigen
    • INCAM 100
    • INCAM-100
    • L1CAM
    • MGC99561
    • V-CAM 1
    • Vascular Cell Adhesion Molecule 1
    • Vascular cell adhesion protein 1
    • VCAM 1
    • VCAM-1
    • VCAM1
    • VCAM1_HUMAN
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100750 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Mouse VCAM1 ELISA Kit 画像

  • Representative Standard Curve using ab100750

  • Representative Standard Curve using ab100750

プロトコール

Mouse VCAM1 ELISA Kit (ab100750) 使用論文

ab100750 has not yet been referenced specifically in any publications.

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The following ELISA kits for mouse ICAM, VCAM, IL-6 and TNF-alpha are suitable for mouse samples:

ab100688 (http://www.abcam.com/ICAM1-Mouse-ELISA-Kit-1-x-96-Well-Plate-ab100688.html)
ab100713 (htt...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"