製品の概要

  • 製品名Mouse Tweak ELISA Kit
    Tweak キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 0.3 pg/ml
  • 検出範囲
    0.27 pg/ml - 200 pg/ml
  • 添加回収試験

    77 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 75.69 68% - 83%
    Serum 79.66 71% - 87%
    Plasma 77.72 70% - 89%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Mouse
  • 製品の概要

    Abcam’s Tweak Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Tweak in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Tweak coated on a 96-well plate. Standards and samples are pipetted into the wells and Tweak present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Tweak antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Tweak bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab113320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Mouse Tweak ELISA Kit 画像

  • Representative Standard Curve using ab113320.

プロトコール

Mouse Tweak ELISA Kit (ab113320) 使用論文

ab113320 has not yet been referenced specifically in any publications.

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This ELISA kit has not been tested against monomeric TWEAK/TNFSF12. It is only known to detect mouse TWEAK in its trimeric form. It is important to note that TWEAK is a homotrimer, mean...

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I am sorry about the problems you have encountered with our kits.

I have emailed the lab to replace the kit component item G (lot GR650572-2) which you had received empty. I will be in touch with more inform...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"