製品の概要

  • 製品名Mouse TrkB ELISA Kit
    TrkB キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Cell Lysate, Tissue Homogenate
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 5 pg/ml
  • 検出範囲
    93.8 pg/ml - 6000 pg/ml
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Mouse
  • 製品の概要

    Abcam’s mouse TrkB in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Mouse TrkB in cell culture supernatants, cell lysates and tissue homogenates.


    A TrkB specific rat monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for TrkB is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse TrkB amount of sample captured in plate.


    For higher sensitivity and reproducibility in only 90 minutes, we recommend Mouse TrkB ELISA Kit (ab203368) from our SimpleStep ELISA® range.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

法規制情報

製品の特性

  • 機能Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1.
  • 組織特異性Isoform TrkB is widely expressed, mainly in the nervous tissue. In the CNS, expression is observed in the cerebral cortex, hippocampus, thalamus, choroid plexus, granular layer of the cerebellum, brain stem, and spinal cord. In the peripheral nervous system, it is expressed in many cranial ganglia, the ophtalmic nerve, the vestibular system, multiple facial structures, the submaxillary glands, and dorsal root ganglia. Isoform TrkB-T1 is expressed in multiple tissues, mainly in brain, pancreas, kidney and heart. Isoform TrkB-T-Shc is predominantly expressed in brain.
  • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 2 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Ligand-mediated auto-phosphorylation.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 別名
    • BDNF tropomyosine receptor kinase B
    • BDNF/NT-3 growth factors receptor
    • GP145-TrkB
    • Neurotrophic tyrosine kinase receptor type 2
    • Ntrk2
    • NTRK2_HUMAN
    • Trk-B
    • TRKB
    • TrkB tyrosine kinase
    • Tyrosine kinase receptor B
    see all
  • 参照データベース

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab119610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Mouse TrkB ELISA Kit 画像

  • Representative standard curve using ab119610

プロトコール

Mouse TrkB ELISA Kit (ab119610) 使用論文

ab119610 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

Unfortunately, this kit cannot be used with rat samples.

We have 2 TrkB antibodies (ab51190 and ab43079) that have been tested in indirect ELISA and rat samples, and might work together in a sandwich EL...

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I'll briefly describe the way our product discount testing program works.

1. Contact Abcam Scientific Support to discuss your novel use of the product
2. If we approve of your testing the product, yo...

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DISCOUNT CODE: ***
Expiration date: June 15th, 2012

I am very pleased to hear you would like to accept our offer and test ab119609 in mouse. This code will give you 1 free ELISA kit before the expiration date. To redeem this offer, pleas...

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Thank you for your patience.

The lab informed me thatRIPA buffer is compatible with the kit and can be usedwithtissue.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"