製品の概要

  • 製品名
    Mouse TNF alpha ELISA Kit
    TNF alpha キット 製品一覧
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 4.5%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 7.1%
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    30 pg/ml
  • 検出範囲
    0.031 ng/ml - 2 ng/ml
  • 添加回収試験

    97 %

  • 全工程の試験時間
    5h 0m
  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Mouse
  • 製品の概要

    Abcam’s TNF alpha Mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of TNF alpha levels in plasma, serum and cell culture supernatants.

    A TNF alpha specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a TNF alpha specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of TNF alpha captured in plate.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

製品の特性

  • 保存方法
    Store at -20°C. Please refer to protocols.
  • 内容 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    50X Biotinylated Mouse TNF alpha Antibody 1 x 140µl
    Chromogen Substrate 1 x 8ml
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
    TNF alpha Microplate (12 x 8 well strips) 1 unit
    TNF alpha Standard 1 vial
  • 研究分野
  • 機能
    Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
  • 関連疾患
    Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
  • 配列類似性
    Belongs to the tumor necrosis factor family.
  • 翻訳後修飾
    The soluble form derives from the membrane form by proteolytic processing.
    The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
    O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
  • 細胞内局在
    Secreted and Cell membrane.
  • Information by UniProt
  • 別名
    • APC1 protein
    • Cachectin
    • DIF
    • soluble form
    • Tnf
    • TNF a
    • TNF macrophage derived
    • TNF monocyte derived
    • TNF superfamily member 2
    • TNF-a
    • TNF-alpha
    • TNFA_HUMAN
    • TNFSF2
    • Tumor necrosis factor
    • Tumor necrosis factor alpha
    • Tumor necrosis factor ligand superfamily member 2
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab108910 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Representative Standard Curve using ab108910

プロトコール

参考文献

This product has been referenced in:
  • Zheng J  et al. Maternal Low-Protein Diet Modulates Glucose Metabolism and Hepatic MicroRNAs Expression in the Early Life of Offspring †. Nutrients 9:N/A (2017). ELISA ; Mouse . Read more (PubMed: 28264458) »

See 1 Publication for this product

レビューと Q&A

The ELISA assays are compatible with RIPA buffer. However, you should prepare your standards and positive controls in the same buffer.

The Bradford assay is compatible with RIPA buffer if the samples are diluted at least 10-fold with distill...

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Thank you for contacting us.

Our kit is color metric not chemoluminescent or fluorescent format. Only those format come with black frame. I don’t think the frame is an issue for the reader.


I hope this information is helpfu...

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Thank you for contacting us.

I am very sorry to hear that you have been having troubles when using this product. In order that I might more fully understand the problem could you please tell me what is sample dilution factor was that you u...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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