製品の概要

  • 製品名Mouse Thrombopoietin ELISA Kit
    Thrombopoietin キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 30 pg/ml
  • 検出範囲
    27.4 pg/ml - 20000 pg/ml
  • 添加回収試験

    94 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 96.23 83% - 103%
    Serum 93.45 82% - 103%
    Plasma 94.28 84% - 104%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Mouse
  • 製品の概要

    Abcam’s Thrombopoietin Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Thrombopoietin in serum, plasma and cell culture supernates.

    This assay employs an antibody specific for Mouse Thrombopoietin coated on a 96-well plate. Standards and samples are pipetted into the wells and Thrombopoietin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Thrombopoietin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Thrombopoietin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 関連性Thrombopoietin is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/cMPL, the product of myeloproliferative leukemia virus oncogene. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
  • 細胞内局在Secreted
  • 別名
    • C mpl ligand
    • Megakaryocyte colony stimulating factor
    • Megakaryocyte growth and development factor
    • Megakaryocyte stimulating factor
    • MGC163194
    • MGDF
    • MKCSF
    • ML
    • MPL ligand
    • MPLLG
    • Myeloproliferative leukemia virus oncogene ligand
    • OTTHUMP00000210504
    • THPO
    • TPO
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100748 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Mouse Thrombopoietin ELISA Kit 画像

  • Representative Standard Curve using ab100748

  • Representative Standard Curve using ab100748

プロトコール

Mouse Thrombopoietin ELISA Kit (ab100748) 使用論文

ab100748 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab100748.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"