Mouse spleen tissue lysate - total protein (ab7937)

製品の概要

  • 製品名Mouse spleen tissue lysate - total protein
    Spleen ライゼート 製品一覧
  • 特記事項Source: White laboratory mice


    Mouse spleen tissue lysate was prepared by homogenization in modified RIPA buffer (150mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris were removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The tissue lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

  • アプリケーション適用あり: WBmore details

法規制情報

製品の特性

  • 保存方法Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: None
    Constituents: 5% Beta mercaptoethanol, 1X SDS
  • Concentration information loading...
  • ライゼート 備考Mouse spleen tissue lysate was prepared by homogenization in modified RIPA buffer (150mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris were removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The tissue lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
  • 研究分野

    アプリケーション

    Our Abpromise guarantee covers the use of ab7937 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    WB Use at an assay dependent concentration. Mouse spleen tissue lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.

    Mouse spleen tissue lysate - total protein (ab7937) 使用論文

    ab7937 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your reply.

    After checking the expression pattern of Ikaros, we would not expect this protein to be detectable in liver samples. As a positive control, I would recommend a spleen whole cell lysate prepared with RIPA buffer (such...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"