Mouse spleen tissue lysate - total protein (ab7937)

製品の概要

  • 製品名
    Mouse spleen tissue lysate - total protein
    Spleen ライゼート 製品一覧
  • 特記事項
    Source: White laboratory mice


    Mouse spleen tissue lysate was prepared by homogenization in modified RIPA buffer (150mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris were removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The tissue lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

  • アプリケーション
    適用あり: WBmore details

法規制情報

製品の特性

  • 保存方法
    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: None
    Constituents: 5% Beta mercaptoethanol, 1X SDS
  • Concentration information loading...
  • ライゼート 備考
    Mouse spleen tissue lysate was prepared by homogenization in modified RIPA buffer (150mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris were removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The tissue lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab7937 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Mouse spleen tissue lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.

参考文献

ab7937 has not yet been referenced specifically in any publications.

レビューと Q&A

Thank you for your reply.

After checking the expression pattern of Ikaros, we would not expect this protein to be detectable in liver samples. As a positive control, I would recommend a spleen whole cell lysate prepared with RIPA buffer (such...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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