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Our Abpromise guarantee covers the use of ab34807 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/20000 - 1/100000.|
|IP||Use at an assay dependent concentration.|
|Immunoelectrophoresis||Use at an assay dependent concentration.|
|ELISA||1/10000 - 1/50000. This product has been assayed against 1.0 µg of Mouse Serum Albumin in a standard indirect ELISA using Peroxidase conjugated Affinity Purified Donkey anti-Rabbit IgG and TMB as a substrate for 30 minutes at room temperature.|
Primary antibody: MSA antibody at 1:1000 in 1% BSA-TTBS for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:20,000 for 45 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting 30 min at 4°C.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"