Anti-Mouse Serum Albumin 抗体 (ab19194)
製品の概要
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製品名Anti-Mouse Serum Albumin antibody
Mouse Serum Albumin 一次抗体 製品一覧 -
製品の詳細Goat polyclonal to Mouse Serum Albumin
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アプリケーション適用あり: ICC, ELISA, WB, IHC-Pmore details
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種交差性交差種: Mouse, Human
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免疫原
This information is considered to be commercially sensitive.
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ポジティブ･コントロール
- This antibody gave a positive signal in human and mouse liver tissue lysates.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPreservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.2 -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab19194 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC | 1/1000 - 1/10000. | |
| ELISA | 1/100 - 1/1000. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa). | |
| IHC-P | Use at an assay dependent dilution. |
ターゲット情報
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機能Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
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組織特異性Plasma.
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配列類似性Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
翻訳後修飾Phosphorylation sites are present in the extracelllular medium.
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細胞内局在Secreted.
- Information by UniProt
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参照データベース
- Entrez Gene: 213 Human
- Entrez Gene: 11657 Mouse
- SwissProt: P02768 Human
- SwissProt: P07724 Mouse
- Unigene: 418167 Human
- Unigene: 16773 Mouse
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別名
- Alb 1 antibody
- alb antibody
- ALBU_MOUSE antibody
see all
Anti-Mouse Serum Albumin antibody 画像
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Western blot - Anti-Mouse Serum Albumin antibody (ab19194)All lanes : Anti-Mouse Serum Albumin antibody (ab19194) at 1 µg/ml
Lane 1 : Liver (Mouse) Tissue Lysate
Lane 2 : Human liver tissue lysate - total protein (ab29889)
Lysates/proteins at 10 µg per lane.
Secondary
Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 69 kDa
Observed band size : 69 kDa
Exposure time : 1 minute -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mouse Serum Albumin antibody (ab19194)Image from Dvorak-Ewell M et al., PLoS One. 2010 Aug 16;5(8):e12194. Fig 7.; doi:10.1371/journal.pone.0012194; August 16, 2010, PLoS ONE 5(8): e12194.ab19194 staining Mouse Serum Albumin (red) in murine liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed with paraformaldehyde and blocked with 1% normal donkey serum, 0.1% bovine serum albumin, 0.1% NaN3, 0.3% Triton X-100 in PBS for 1 hour at room temperature; antigen retrieval was using a citrate buffer. Samples were incubated with primary antibody and an AlexaFluor®555-conjugated IgG was used as the secondary antibody.
プロトコール
Anti-Mouse Serum Albumin antibody (ab19194) 使用論文
This product has been referenced in:
- Zhang H et al. Functional liver tissue engineering by an adult mouse liver-derived neuro-glia antigen 2-expressing stem/progenitor population. J Tissue Eng Regen Med N/A:N/A (2016). Read more (PubMed: 27638002) »
- Singh S et al. Physiologic variations in blood plasminogen levels affect outcomes after acute cerebral thromboembolism in mice: a pathophysiologic role for microvascular thrombosis. J Thromb Haemost 14:1822-32 (2016). Read more (PubMed: 27319402) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"