製品の概要

  • 製品名
    Anti-Mouse Serum Albumin antibody
    Mouse Serum Albumin 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Mouse Serum Albumin
  • 由来種
    Goat
  • アプリケーション
    適用あり: ICC, ELISA, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    This information is considered to be commercially sensitive.

  • ポジティブ･コントロール
    • This antibody gave a positive signal in human and mouse liver tissue lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab19194 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC 1/1000 - 1/10000.
ELISA 1/100 - 1/1000.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
IHC-P Use at an assay dependent dilution.

ターゲット情報

  • 機能
    Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
  • 組織特異性
    Plasma.
  • 配列類似性
    Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • 翻訳後修飾
    Phosphorylation sites are present in the extracelllular medium.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
    • 別名
      • Alb 1 antibody
      • alb antibody
      • ALBU_MOUSE antibody
      • Albumin 1 antibody
      • Albumin antibody
      • Serum albumin antibody
      see all

    画像

    • Western blot - Anti-Mouse Serum Albumin antibody (ab19194)
      Western blot - Anti-Mouse Serum Albumin antibody (ab19194)
      All lanes : Anti-Mouse Serum Albumin antibody (ab19194) at 1 µg/ml

      Lane 1 : Liver (Mouse) Tissue Lysate
      Lane 2 : Human liver tissue lysate - total protein (ab29889)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 69 kDa
      Observed band size: 69 kDa


      Exposure time: 1 minute
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mouse Serum Albumin antibody (ab19194)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mouse Serum Albumin antibody (ab19194)Image from Dvorak-Ewell M et al., PLoS One. 2010 Aug 16;5(8):e12194. Fig 7.; doi:10.1371/journal.pone.0012194; August 16, 2010, PLoS ONE 5(8): e12194.
      ab19194 staining Mouse Serum Albumin (red) in murine liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

      Tissue was fixed with paraformaldehyde and blocked with 1% normal donkey serum, 0.1% bovine serum albumin, 0.1% NaN3, 0.3% Triton X-100 in PBS for 1 hour at room temperature; antigen retrieval was using a citrate buffer. Samples were incubated with primary antibody and an AlexaFluor®555-conjugated IgG was used as the secondary antibody.

    参考文献

    This product has been referenced in:

    See all 26 Publications for this product

    ab19194 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

    レビューを送る 質問を送る

    5 reviews or Q&A

    10
    Votes: Highest First

    Our customer is interested in using one of the following antibodies for the detection of albmin in mouse serum by ELISA.

    http://www.abcam.com/Mouse-Serum-Albumin-antibody-ab19194.html
    http://www.abcam.com/Mouse-Serum-Albumin-antibody-HRP-a...

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    Thank you for contacting us.
    As we haven't tested the cross-reactivity for this antibodies, it is very much likely that they will react with other albumins.
    Therefore, I would suggest to use milk as a blocking agent (0.5% Skim milk/PBS for 1 h...

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    I used the antibody in the meantime and it worked fine with mouse serum, but not with my target protein. I understood that abcam does not want to disclose the immunogen but given my negative result it would be very important for me to know if the bindi...

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    Thank you for your response.

    It would be much appreciated if I could get some more information which would help me identify the source of the problem. Could you provide some further details of the protocol used and complete the following form...

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    Which blocking agent do you recommend for anti-mouse albumin antibody ab19194? SECONDARY ANTIBODY which dilution buffer do you recommend for the secondary antibody for ab19194?

    Thank you for your enquiry regarding ab19194 and your interest in our products.

    I can confirm that we have used BSA as our blocking agent for our in-house testings.

    I hope this helps and if I can assist further, please do not hesita...

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    I would like to know what antibodies you have available as Western Blot loading controls for mouse serum. Antibodies against general mouse Ig have been moderately successful but we would like to consider other options.

    Thank you for contacting us. Serum albumin may be a suitable loading control, but I am not sure if levels will vary from one animal to another, for example healthy vs. diseased. These three antibodies detect mouse serum albumin: ab19194...

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    I have a customer who has just purchased AB19194.  She would like to store this antibody in the freezer, can you please let me know if this antibody can be stored frozen?

    Thank you for your inquiry. I would not recommend storing this antibody frozen. We have not tested this antibody after it's been frozen so we can't say how it would affect the stability of the antibody. Plus, this antibody does not have any cryopr...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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