製品の概要

  • 製品名Mouse SDF1 ELISA Kit (CXCL12)
    SDF1 alpha キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 3 pg/ml
  • 検出範囲
    9.38 pg/ml - 600 pg/ml
  • 添加回収試験

    99 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 99.34 89% - 107%
    Serum 98.76 88% - 106%
    Plasma 100.36 90% - 107%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Mouse
  • 製品の概要

    Abcam’s SDF1 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse SDF1 in serum (mouse SDF1 concentration is pretty low in normal serum/plasma, it may not be detected in this assay), plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse SDF1 coated on a 96-well plate. Standards and samples are pipetted into the wells and SDF1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse SDF1antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SDF1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 関連性SDF1 alpha and 1 beta are small cytokines that belong to the intercrine family, members of which activate leukocytes and are often induced by proinflammatory stimuli such as lipopolysaccharide, TNF or IL1. SDF1 alpha has been shown to be chemotactic for lymphocytes and is recently reported to be a ligand for CXCR4 (LESTR/fusin), a co-receptor for HIV1 entry into T cells. SDF-1 binding to CXCR4 inhibits HIV1 entry.
  • 別名
    • C-X-C motif chemokine 12
    • Chemokine (C X C motif) ligand 12
    • CXCL12
    • hIRH
    • Intercrine reduced in hepatomas
    • IRH
    • PBSF
    • Pre B cell growth stimulating factor
    • SCYB12
    • SDF 1
    • SDF 1 beta
    • SDF 1b
    • SDF1
    • SDF1 alpha (3-67)
    • SDF1 beta (3-72)
    • SDF1A
    • SDF1B
    • Stromal cell derived factor 1
    • TLSF a
    • TLSF b
    • TLSFa
    • TLSFb
    • TPAR1
    see all

アプリケーション

Our Abpromise guarantee covers the use of ab100741 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Mouse SDF1 ELISA Kit (CXCL12) 画像

  • SDF1 measured in culture supernatants from control or activated cells (P/L), stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297) with the addition of 1 ug x mL-1 of LPS (Sigma) for the last 6 hours, and in biological fluids (duplicates +/- SD). All samples tested undiluted.

  • Standard curve with background signal subtracted (duplicates; +/- SD).

  • Representative Standard Curve using ab100741

  • Representative Standard Curve using ab100741

プロトコール

Mouse SDF1 ELISA Kit (CXCL12) (ab100741) 使用論文

This product has been referenced in:
  • Shi W  et al. Transplantation of RADA16-BDNF peptide scaffold with human umbilical cord mesenchymal stem cells forced with CXCR4 and activated astrocytes for repair of traumatic brain injury. Acta Biomater 45:247-261 (2016). Read more (PubMed: 27592818) »

See 1 Publication for this product

Product Wall

Mouse SDF-1 ELISA

Good Excellent 5/5 (Ease of Use)
Abreviews
I used the Mouse SDF-1 ELISA kit to check the levels in mouse retinal tissues and it worked.
Username

Dr. Finny Monickaraj

Verified customer

投稿 Aug 19 2015

Both antibodies used in this kit recognize a region within Lys22­ - Lys89 (Accession # Q6ICW0). Thus, although not previously tested, we do not expect an additional sequenced fused to the C-terminus to interfere with detection.

Thank you for your reply.

You may want to try the new lots and take the precautions listed in the previous email. The lab does not think that anything is wrong with the standards as all lots are quality control tested. Just take the necessary...

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Thank you for your inquiry and your patience while I looked into this further.

The lab has QC'ed these standards for the kit and the certificate of analysis for a recent batch is attached.

The150 rpm is a little fast but it is not l...

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Thank you for your reply.

Just to clarify, the diluted/working standards (600, 200, 66.7, 22.2, 7.4, 2.5, 0.82 pg/ml) should not be stored. The reconstituted standard (50 ng/ml) can be stored at -80ºC for 1-2 weeks at most. Remember, an ...

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Thank you very much for your email.

I am sorry to hear about the customer who has decided to pull out from getting this product. We have helped your customer as far as we can by providing them credit note which you have used for order 112737. ...

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Thank you for your inquiry.

No, the protocol is right. Steps 4 and 5 are not the same as steps 6 and 7. Steps 4 and 5 involve the biotinylated antibody and steps 6 and 7 involved the streptavidin-HRP solution.

Yes, step 6 in "A...

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Thank you for your inquiry.

We know of the IL6 ELISA kit being tested in liver tissue lysate, but unfortunately we do not have any info on brain lysate.

For the SDF1 alpha ELISA kit, we haven't tested tissues with this kit but it wou...

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After reviewing the standard OD data, it doesn’t look like there is any significant detection or linear pattern. Did you run any samples as this would help determine if the issue is just with the standard protein or a common detection component? Stri...

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Thank you for contacting us. I am happy to offer you some tips to help you improve better results from this kit. I would also appreciate if you could send me the raw OD data, so we can try to find out why the standards provided such low values. ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"