製品の概要

  • 製品名Mouse Resistin ELISA kit (RETN)
    Resistin キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 3.1%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 7.2%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    = 0.04 ng/ml
  • 検出範囲
    0.063 ng/ml - 4 ng/ml
  • 添加回収試験

    97 %

  • 全工程の試験時間
    5h 00m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Mouse
  • 製品の概要

    Abcam’s Resistin (RETN) Mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Resistin  levels in plasma, serum and cell culture supernatants.

    A Resistin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Resistin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Resistin captured in plate.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Hormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes.
  • 組織特異性Expressed only in fatty tissues.
  • 配列類似性Belongs to the resistin/FIZZ family.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • Adipose tissue specific secretory factor
    • Adipose tissue-specific secretory factor
    • ADSF
    • C/EBP epsilon regulated myeloid specific secreted cysteine rich protein
    • C/EBP epsilon regulated myeloid specific secreted cysteine rich protein precursor 1
    • C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein
    • Cysteine rich secreted protein A12 alpha like 2
    • Cysteine rich secreted protein FIZZ3
    • Cysteine-rich secreted protein A12-alpha-like 2
    • Cysteine-rich secreted protein FIZZ3
    • FIZZ 3
    • FIZZ3
    • Found in inflammatory zone 3
    • HXCP 1
    • HXCP1
    • MGC126603
    • MGC126609
    • PRO1199
    • Resistin
    • Resistin delta2
    • RETN
    • RETN 1
    • RETN_HUMAN
    • RETN1
    • UNQ407
    • XCP 1
    • XCP1
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab108900 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Mouse Resistin ELISA kit (RETN) 画像

  • Representative Standard Curve using ab108900

プロトコール

Mouse Resistin ELISA kit (RETN) (ab108900) 使用論文

ab108900 has not yet been referenced specifically in any publications.

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