Mouse RANKL ELISA Kit (TNFSF11) (ab100749)

製品の概要

  • 製品名Mouse RANKL ELISA Kit (TNFSF11)
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 4 pg/ml
  • 検出範囲
    2.74 pg/ml - 2000 pg/ml
  • 添加回収試験

    96 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 106.5 95% - 115%
    Serum 95.05 83% - 104%
    Plasma 89.2 74% - 104%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rat, Human, Pig
  • 製品の概要

    Abcam’s RANKL Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse RANKL in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Mouse RANKL coated on a 96- well plate. Standards and samples are pipetted into the wells and RANKL present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse RANKL antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of RANKL bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.
  • 組織特異性Highest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid.
  • 関連疾患Defects in TNFSF11 are the cause of osteopetrosis autosomal recessive type 2 (OPTB2) [MIM:259710]; also known as osteoclast-poor osteopetrosis. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Autosomal recessive osteopetrosis is usually associated with normal or elevated amount of non-functional osteoclasts. OPTB2 is characterized by paucity of osteoclasts, suggesting a molecular defect in osteoclast development.
  • 配列類似性Belongs to the tumor necrosis factor family.
  • 翻訳後修飾The soluble form of isoform 1 derives from the membrane form by proteolytic processing (By similarity). The cleavage may be catalyzed by ADAM17.
  • 細胞内局在Cytoplasm; Secreted and Cell membrane.
  • Information by UniProt
  • 別名
    • CD254
    • CD254 antigen
    • hRANKL2
    • ODF
    • OPGL
    • OPTB2
    • Osteoclast differentiation factor
    • Osteoprotegerin ligand
    • RANKL
    • receptor activator of nuclear factor kappa B ligand
    • Receptor activator of nuclear factor kappa-B ligand
    • sOdf
    • soluble form
    • TNF-related activation-induced cytokine
    • TNF11_HUMAN
    • Tnfsf11
    • TRANCE
    • tumor necrosis factor (ligand) superfamily, member 11
    • Tumor necrosis factor ligand superfamily member 11
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100749 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Mouse RANKL ELISA Kit (TNFSF11) 画像

  • RANKL measured in biological fluids showing quantity (pg) per mL of tested sample

  • Representative Standard Curve using ab100749

  • Representative Standard Curve using ab100749

プロトコール

Mouse RANKL ELISA Kit (TNFSF11) (ab100749) 使用論文

ab100749 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. I have had the opportunity to discuss your question with the lab.

This product has to our knowledge not been tested on human serum samples. However the sequence homology between the two species is such that it is...

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The 20X Wash Buffer contained in this kit is available for purchase in our catalogue under reference number http://www.abcam.com/ab173280.html.

Abreview for RANKL Mouse ELISA Kit

Good Good 4/5 (Ease of Use)
Abreviews

Sample: Mouse Serum (Test with human serum to see specificity)
Specification: Test with human serum to see specificity
Amount of Sample: 25 ul each µg
Type of Kit Used: ELISA
Positive control: Positive controls are mice serum, the RANKL level is 0-200 pg/ml, the human serum I tested with this kit is 140 pg/ml, so this kit can also be used for human samples, not specific for mouse.
Did you use the Abcam Kit protocol?: Yes
Username

Mr. GJ Xia

Verified customer

投稿 Jan 11 2013

Thank you for your email.

Yes, we do have OPG kits available. The catalogue number is ab100617 and ab100733, one is specific to human and other one to mouse.

http://www.abcam.com/Osteoprotegerin-Human-ELISA-Kit-1-x-96-Well-Plate-ab100...

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Thank you for contacting us.

Could you please provide the species you are interested in? We have mouse RANKL ELISA kit to use for serum as well as plasma.

http://www.abcam.com/RANKL-mouse-ELISA-Kit-1-x-96-Well-Plate-ab100749.html
...

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I am very pleased to hear you would like to accept our offer and test ab100749 in human. This code will give you: $470 off a future order before the expiration date. To redeem this offer, please submit an Abreview forhuman and include this code in the ...

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Thank you very much for your interest in ab100749.

To our knowledge, ab100749 has not been tested in human. Therefore, I can offer a discount off a future purchase if you buy ab100749 now, test it inhuman and submit feedback to us in the form ...

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Thank you for contacting Abcam regarding ab100749.


I have confirmed with the laboratory that EDTA will not interfere with this kit.When we tested normal mouse serum and plasma samples with this kit, the measured levels were around 300 pg/...

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Thank you for sending along the data.

In both graphs the concentrations are listed in log scale but the ODs are shown in linear scale. The ODs should also be in log scale on the y axis.

The OPG data looks as expected, where the conc...

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Thank you for calling us on Friday.

I can now confirm to you that the wash buffers of the two kits (ab100733 and ab100749) are interchangeable and if you want you could just make up one for one kit and use with both kits until you need to mak...

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