Mouse RANKL ELISA Kit (TNFSF11) (ab100749)
Key features and details
- Sensitivity: 4 pg/ml
- Range: 2.74 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
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製品名
Mouse RANKL ELISA Kit (TNFSF11)
RANKL キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 4 pg/ml -
検出範囲
2.74 pg/ml - 2000 pg/ml -
添加回収試験
96 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 106.5 95% - 115% Serum 95.05 83% - 104% Plasma 89.2 74% - 104% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Mouse
交差が予測される動物種: Rat, Human, Pig -
製品の概要
Abcam’s RANKL Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse RANKL in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Mouse RANKL coated on a 96- well plate. Standards and samples are pipetted into the wells and RANKL present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse RANKL antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of RANKL bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Mouse RANKL 2 vials RANKL Microplate (12 strips x 8 wells) 1 unit Recombinant Mouse RANKL Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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機能
Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. -
組織特異性
Highest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid. -
関連疾患
Defects in TNFSF11 are the cause of osteopetrosis autosomal recessive type 2 (OPTB2) [MIM:259710]; also known as osteoclast-poor osteopetrosis. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Autosomal recessive osteopetrosis is usually associated with normal or elevated amount of non-functional osteoclasts. OPTB2 is characterized by paucity of osteoclasts, suggesting a molecular defect in osteoclast development. -
配列類似性
Belongs to the tumor necrosis factor family. -
翻訳後修飾
The soluble form of isoform 1 derives from the membrane form by proteolytic processing (By similarity). The cleavage may be catalyzed by ADAM17. -
細胞内局在
Cytoplasm; Secreted and Cell membrane. - Information by UniProt
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別名
- CD254
- hRANKL2
- ODF
see all -
参照データベース
- Entrez Gene: 8600 Human
- Entrez Gene: 21943 Mouse
- Entrez Gene: 117516 Rat
- Omim: 602642 Human
- SwissProt: O14788 Human
- SwissProt: O35235 Mouse
- SwissProt: Q9ESE2 Rat
- Unigene: 333791 Human
see all
画像
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (21)
ab100749 は 21 報の論文で使用されています。
- Liu Y et al. An optogenetic approach for regulating human parathyroid hormone secretion. Nat Commun 13:771 (2022). PubMed: 35140213
- Arra M et al. I?B-? signaling promotes chondrocyte inflammatory phenotype, senescence, and erosive joint pathology. Bone Res 10:12 (2022). PubMed: 35145063
- Zhang X et al. Bone-targeted nanoplatform enables efficient modulation of bone tumor microenvironment for prostate cancer bone metastasis treatment. Drug Deliv 29:889-905 (2022). PubMed: 35285760
- Hu Y et al. RANKL from bone marrow adipose lineage cells promotes osteoclast formation and bone loss. EMBO Rep 22:e52481 (2021). PubMed: 34121311
- Lee GL et al. Restoration of 5-methoxytryptophan protects against atherosclerotic chondrogenesis and calcification in ApoE-/- mice fed high fat diet. J Biomed Sci 28:74 (2021). PubMed: 34749728