製品の概要

  • 製品名Mouse RANK ELISA Kit (CD265)
    RANK キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 2 pg/ml
  • 検出範囲
    62.5 pg/ml - 4000 pg/ml
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Mouse
  • 製品の概要

    Abcam’s Mouse RANK (CD265) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of mouse RANK (CD265) in cell culture supernatants and serum. 

    A RANK (CD265) specific rat monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for RANK is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse RANK amount of sample captured in plate.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

法規制情報

製品の特性

  • 機能Receptor for TNFSF11/RANKL/TRANCE/OPGL; essential for RANKL-mediated osteoclastogenesis. Involved in the regulation of interactions between T-cells and dendritic cells.
  • 組織特異性Ubiquitous expression with high levels in skeletal muscle, thymus, liver, colon, small intestine and adrenal gland.
  • 関連疾患Defects in TNFRSF11A are the cause of familial expansile osteolysis (FEO) [MIM:174810]. FEO is a rare autosomal dominant bone disorder characterized by focal areas of increased bone remodeling. The osteolytic lesions develop usually in the long bones during early adulthood. FEO is often associated with early onset deafness and loss of dentition.
    Defects in TNFRSF11A are a cause of Paget disease of bone type 2 (PDB2) [MIM:602080]; also known as familial Paget disease of bone. PDB2 is a bone-remodeling disorder with clinical similarities to FEO. Unlike FEO, however, affected individuals have involvement of the axial skeleton with lesions in the spine, pelvis and skull.
    Defects in TNFRSF11A are the cause of osteopetrosis autosomal recessive type 7 (OPTB7) [MIM:612301]; also called osteoclast-poor osteopetrosis with hypogammaglobulinemia. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. OPTB7 is characterized by paucity of osteoclasts, suggesting a molecular defect in osteoclast development. OPTB7 is associated with hypogammaglobulinemia.
  • 配列類似性Contains 4 TNFR-Cys repeats.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 別名
    • CD265
    • FEO
    • LOH18CR1
    • Loss of heterozygosity 18 chromosomal region 1
    • ODFR
    • OFE
    • OPTB7
    • Osteoclast differentiation factor receptor
    • OSTS
    • PDB2
    • Receptor activator of NF-KB
    • Receptor activator of nuclear factor kappa B
    • TNFRSF11A
    • TNR11_HUMAN
    • TRANCER
    • Tumor necrosis factor receptor superfamily member 11A
    • Tumor necrosis factor receptor superfamily member 11a activator of NFKB
    • Tumor necrosis factor receptor superfamily member 11a NFKB activator
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab119606 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Mouse RANK ELISA Kit (CD265) 画像

  • Representative standard curve using ab119606

プロトコール

Mouse RANK ELISA Kit (CD265) (ab119606) 使用論文

ab119606 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your email.

Yes, we do have OPG kits available. The catalogue number is ab100617 and ab100733, one is specific to human and other one to mouse.

http://www.abcam.com/Osteoprotegerin-Human-ELISA-Kit-1-x-96-Well-Plate-ab100...

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Thank you for contacting us.

Could you please provide the species you are interested in? We have mouse RANKL ELISA kit to use for serum as well as plasma.

http://www.abcam.com/RANKL-mouse-ELISA-Kit-1-x-96-Well-Plate-ab100749.html
...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"