製品の概要

  • 製品名Mouse RAGE ELISA Kit
    RAGE キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 15 pg/ml
  • 検出範囲
    13.72 pg/ml - 10000 pg/ml
  • 添加回収試験

    87 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 75.89 67% - 82%
    Serum 102.9 91% - 110%
    Plasma 82.69 71% - 92%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Mouse
  • 製品の概要

    Abcam’s RAGE Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse RAGE in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse RAGE coated on a 96-well plate. Standards and samples are pipetted into the wells and RAGE present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse RAGE antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of RAGE bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space.
  • 組織特異性Endothelial cells.
  • 配列類似性Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 細胞内局在Secreted and Cell membrane.
  • Information by UniProt
  • 別名
    • Advanced glycosylation end product-specific receptor
    • Ager
    • MGC2235
    • RAGE_HUMAN
    • Receptor for advanced glycosylation end products
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100738 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Mouse RAGE ELISA Kit 画像

  • Standard curve of mouse RAGE with background signal subtracted (duplicates; +/- SD).

  • Mouse RAGE measured in in mouse tissue lysates (in RIPA buffer tested in the range of 0.1ug to 0.1 mg protein, quantity of RAGE shown as per mg of extracted protein ), duplicates +/- SD.

  • Mouse RAGE measured in biological fluids (dilution range neat - 1/10; duplicates +/- SD).

  • Representative standard curve using ab100738

プロトコール

Mouse RAGE ELISA Kit (ab100738) 使用論文

ab100738 has not yet been referenced specifically in any publications.

Product Wall

Tha lab sent their data for the two lots that gave you low standard OD compared to the June assay.

October


Standard (pg/ml) OD
10,000 2.832
3,333 1.512
1,111 0.732
370.4 0.351
123.5 0.201
41.15 0....

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Thank you for contacting us.

The recommended dilution for testing normal mouse serum and plasma with this kit is 2-fold.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or in...

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Thank you for contacting us.  This ELISA kit has not been validated for use on tissue samples, but may be suitable for that purpose.  Theoretically, any low-salt lysis buffer that meets the following criteria should be compatible with this kit: ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"