製品の概要
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製品名Mouse RAGE ELISA Kit
RAGE キット 製品一覧 -
検出方法Colorimetric
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サンプルの種類Cell culture supernatant, Serum, Plasma
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アッセイタイプSandwich (quantitative)
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検出感度< 15 pg/ml
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検出範囲13.72 pg/ml - 10000 pg/ml
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添加回収試験
87 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 75.89 67% - 82% Serum 102.9 91% - 110% Plasma 82.69 71% - 92% -
ステップMultiple steps standard assay
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種交差性交差種: Mouse
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製品の概要
Abcam’s RAGE Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse RAGE in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse RAGE coated on a 96-well plate. Standards and samples are pipetted into the wells and RAGE present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse RAGE antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of RAGE bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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アプリケーション適用あり: Sandwich ELISAmore details
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試験プラットフォームMicroplate
製品の特性
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保存方法Store at -20°C. Please refer to protocols.
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内容 1 x 96 tests 160X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent 1 x 15ml Biotinylated anti-mouse RAGE (lyophilized) 2 vials RAGE Microplate (12 x 8 wells) 1 unit Recombinant Mouse RAGE Standard 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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機能Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space.
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組織特異性Endothelial cells.
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配列類似性Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
細胞内局在Secreted and Cell membrane.
- Information by UniProt
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別名
- Advanced glycosylation end product-specific receptor
- Ager
- MGC2235
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参照データベース
- Entrez Gene: 11596 Mouse
- SwissProt: Q62151 Mouse
- Unigene: 3383 Mouse
アプリケーション
Our Abpromise guarantee covers the use of ab100738 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
画像
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ELISA: RAGE Mouse ELISA Kit (ab100738)Standard curve of mouse RAGE with background signal subtracted (duplicates; +/- SD).
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ELISA: RAGE Mouse ELISA Kit (ab100738)Mouse RAGE measured in in mouse tissue lysates (in RIPA buffer tested in the range of 0.1ug to 0.1 mg protein, quantity of RAGE shown as per mg of extracted protein ), duplicates +/- SD.
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ELISA: RAGE Mouse ELISA Kit (ab100738)Mouse RAGE measured in biological fluids (dilution range neat - 1/10; duplicates +/- SD).
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Typical standard curveRepresentative standard curve using ab100738
プロトコール
参考文献
ab100738 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"