製品の概要

  • 製品名Mouse PIWIL4 peptide

製品の詳細

  • 由来Synthetic
  • アミノ酸配列
    • アクセッション番号Q8CGT6
    • 生物種Mouse
    • 分子量95 kDa

関連製品

特性

Our Abpromise guarantee covers the use of ab23535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Blocking - Blocking peptide for Anti-PIWIL4 antibody (ab21869)

  • 製品の状態Liquid
  • 備考

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

関連情報

  • 別名
    • DKFZp686P01248
    • FLJ36156
    • HILI 2
    • HILI2
    • HIWI 2
    • HIWI2
    • Miwi 2 protein
    • Miwi2
    • PIWI
    • Piwi like 2
    • Piwi like 4
    • Piwi like 4 (Drosophila)
    • PIWI like protein
    • Piwi like protein 4
    • Piwi like RNA mediated gene silencing 4
    • Piwi-like protein 4
    • PIWIL 4
    • Piwil4
    • PIWL4_HUMAN
    see all
  • 機能Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with secondary piRNAs antisense and PIWIL2/MILI is required for such association. The piRNA process acts upstream of known mediators of DNA methylation. Participates to a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation (By similarity). May be involved in the chromatin-modifying pathway by inducing 'Lys-9' methylation of histone H3 at some loci.
  • 組織特異性Expressed in testis. According to PubMed:17544373, it is ubiquitously expressed.
  • 配列類似性Belongs to the argonaute family. Piwi subfamily.
    Contains 1 PAZ domain.
    Contains 1 Piwi domain.
  • 翻訳後修飾Arginine methylation by PRMT5 is required for the interaction with Tudor domain-containing protein (TDRD1, TDRKH/TDRD2 and TDRD9) and subsequent localization to the meiotic nuage, also named P granule.
  • 細胞内局在Nucleus. Cytoplasm. Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis. PIWIL2/MILI is required for nuclear localization.
  • Information by UniProt

Mouse PIWIL4 peptide 画像

  • Lanes 1 - 2 : Anti-PIWIL4 antibody (ab21869) at 2 µg/ml
    Lanes 3 - 4 : Anti-PIWIL4 antibody (ab21869) at 1 µg/ml

    Lane 1 : Testis (Mouse) Tissue Lysate - normal tissue (ab4027)
    Lane 2 : Testis (Mouse) Tissue Lysate - normal tissue (ab4027) with Mouse PIWIL4 peptide (ab23535) at 1 µg/ml
    Lane 3 : Testis (Mouse) Tissue Lysate - normal tissue (ab4027)
    Lane 4 : Testis (Mouse) Tissue Lysate - normal tissue (ab4027) with Mouse PIWIL4 peptide (ab23535) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    Rabbit IgG secondary antibody (ab28446) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 98 kDa
    Observed band size : 98 kDa

    See Abreview

Mouse PIWIL4 peptide (ab23535) 使用論文

ab23535 has not yet been referenced specifically in any publications.

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