製品の概要

  • 製品名Mouse PF4 ELISA Kit (CXCL4)
    PF4 キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 6 pg/ml
  • 検出範囲
    4.12 pg/ml - 3000 pg/ml
  • 添加回収試験

    100 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 73.99 67% - 83%
    Serum 118.2 104% - 127%
    Plasma 109.3 92% - 129%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Mouse
  • 製品の概要

    Abcam’s PF4 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse PF4 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse PF4 coated on a 96-well plate. Standards and samples are pipetted into the wells and PF4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse PF4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PF4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form.
  • 配列類似性Belongs to the intercrine alpha (chemokine CxC) family.
  • 翻訳後修飾Binds non-covalently to a proteoglycan molecule.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • C-X-C motif chemokine 4
    • Chemokine (C X C motif) ligand 4
    • Chemokine (CXC motif) ligand 4
    • CXCL 4
    • CXCL4
    • Iroplact
    • MGC138298
    • Oncostatin A
    • Oncostatin-A
    • OncostatinA
    • PF 4
    • PF-4
    • PF4
    • Platelet factor 4
    • PLF4_HUMAN
    • SCYB 4
    • SCYB4
    • short form
    • Small inducible cytokine subfamily member 4
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100735 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Mouse PF4 ELISA Kit (CXCL4) 画像

  • PF4 measured in cell supernatanses showing quantity (pg) per mL tested sample

  • PF4 measured in biofluids showing quantity (microgram) per mL of tested sample

  • Representative standard curve using ab100735

  • Representative standard curve using ab100735

プロトコール

Mouse PF4 ELISA Kit (CXCL4) (ab100735) 使用論文

This product has been referenced in:
  • Petzold T  et al. ß1 integrin-mediated signals are required for platelet granule secretion and hemostasis in mouse. Blood 122:2723-31 (2013). Mouse . Read more (PubMed: 24004668) »

See 1 Publication for this product

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The lab let me know that sodium citrate is also an acceptable anti-coagulant.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"