Mouse PD1 ELISA Kit (ab210971)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 21 pg/ml
- Range: 125 pg/ml - 8000 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
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製品名
Mouse PD1 ELISA Kit
PD1 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% EL-4 ext 5 4% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% EL-4 ext 3 7% -
サンプルの種類
Cell culture supernatant, Cell culture extracts, Tissue Extracts -
アッセイタイプ
Sandwich (quantitative) -
検出感度
21 pg/ml -
検出範囲
125 pg/ml - 8000 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 100 93% - 104% Cell culture extracts 109 100% - 115% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Mouse -
製品の概要
Mouse PD1 ELISA Kit (ab210971) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PD1 protein in cell culture extracts, cell culture supernatant, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse PD1 with 21 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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特記事項
PD1 is a single-pass type I cell membrane glycoprotein. It is a receptor for PD-1L and/or PD-L2 ligands. PD1 is involved in the regulation of T-cell function during immunity and tolerance. PD-1 interactions with its ligands PD-L1 and/or PD-L2 provides a negative regulatory signal to CD4 and CD8 T cells that results ultimately in a phenotype termed T cell exhaustion. Possible cell death inducer, in association with other factors.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Mouse PD-1 Capture Antibody 1 x 600µl 10X Mouse PD-1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPR 1 x 6ml Denaturant 1 x 500µl Mouse PD-1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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機能
Possible cell death inducer, in association with other factors. -
関連疾患
Genetic variation in PDCD1 is associated with susceptibility to systemic lupus erythematosus type 2 (SLEB2) [MIM:605218]. Systemic lupus erythematosus is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. -
配列類似性
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
発生段階
Induced at programmed cell death. -
細胞内局在
Membrane. - Information by UniProt
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別名
- CD279
- CD279 antigen
- hPD 1
see all -
参照データベース
- Entrez Gene: 18566 Mouse
- SwissProt: Q02242 Mouse
- Unigene: 5024 Mouse
関連製品
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Alternative Versions
画像
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Background-subtracted data values (mean +/- SD) are graphed.
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Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of PD1 were measured in duplicate and interpolated from the PD1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PD1 concentration was determined to be 4,246 pg/mL in EL-4 cell extract.
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The concentrations of PD1 were measured in duplicates, interpolated from the PD1 standard curves and corrected for sample dilution. Undiluted samples are as follows: EL-4 cell culture supernatant 100%. Dilutions beyond 4 fold were not analyzed due to O.D. values below the O.D. values of the MDD. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PD1 concentration was determined to be 195 pg/mL in EL-4 cell culture supernatant samples.
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EL-4 cells were grown in the absence or presence of PHA/PMA for 48 hours. The concentrations of PD1 were measured in neat supernatant samples in duplicates and interpolated from the PD1 standard curve. The interpolated values are plotted (mean +/- SD, n=2). The mean PD1 concentration was determined to be 32 pg/mL in unstimulated, 203 pg/ml in PHA/PMA stimulated supernatants and undetectable in media (not shown).
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To learn more about the advantages of recombinant antibodies see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab210971 は 1 報の論文で使用されています。
- Su J et al. Antitumor Activity of Extract From the Sporoderm-Breaking Spore of Ganoderma lucidum: Restoration on Exhausted Cytotoxic T Cell With Gut Microbiota Remodeling. Front Immunol 9:1765 (2018). PubMed: 30108589