製品の概要

  • 製品名Mouse Osteopontin ELISA Kit
    Osteopontin キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 4 pg/ml
  • 検出範囲
    2.74 pg/ml - 2000 pg/ml
  • 添加回収試験

    100 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 85.46 78% - 105%
    Serum 115.6 103% - 122%
    Plasma 110.4 87% - 130%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Mouse
  • 製品の概要

    Abcam’s Osteopontin Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Osteopontin in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Osteopontin coated on a 96-well plate. Standards and samples are pipetted into the wells and Osteopontin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Osteopontin antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Osteopontin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
    Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
  • 組織特異性Bone. Found in plasma.
  • 配列類似性Belongs to the osteopontin family.
  • 翻訳後修飾Extensively phosphorylated on clustered serine residues.
    N- and O-glycosylated.
    Phosphorylation sites are present in the extracelllular medium.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • BNSP
    • Bone sialoprotein 1
    • BSP I
    • BSPI
    • Early T lymphocyte activation 1
    • ETA 1
    • ETA1
    • MGC110940
    • Nephropontin
    • OPN
    • Osteopontin
    • osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein
    • OSTP_HUMAN
    • PSEC0156
    • Secreted phosphoprotein 1
    • secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1)
    • SPP 1
    • SPP-1
    • SPP1
    • SPP1/CALPHA1 fusion
    • Urinary stone protein
    • Uropontin
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Mouse Osteopontin ELISA Kit 画像

  • Representative standard curve using ab100734

プロトコール

Mouse Osteopontin ELISA Kit (ab100734) 使用論文

This product has been referenced in:
  • Wong TM  et al. Respiratory syncytial virus (RSV) infection in elderly mice results in altered antiviral gene expression and enhanced pathology. PLoS One 9:e88764 (2014). Read more (PubMed: 24558422) »
  • Fang X  et al. Ameliorated ConA-induced hepatitis in the absence of PKC-theta. PLoS One 7:e31174 (2012). Mouse . Read more (PubMed: 22347449) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us.

The mouse OPN kit does have a very low level cross-reactivity with human OPN. We’ve measure it to around 2-3% cross-reactivity. This is low enough I think you could measure it in your sample, but you might ne...

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Abreview for Osteopontin Mouse ELISA Kit

Good Good 4/5 (Ease of Use)
Abreviews

Sample: Mouse (Lavage fluid)
Specification: Lavage fluid
Amount of Sample: Dilutions of pooled lavage µg
Type of Kit Used: ELISA
Positive control: recombinant Osteopontin spiked in diluent
Did you use the Abcam Kit protocol?: Yes
Username

Abcam user community

Verified customer

投稿 Mar 18 2013

Thank youfor your inquiry.

The quote for ab8448 has been send in a separate email.

I am happy to confirm that we offer ELISA kits for Osteopontin:

ab100618 (Osteopontin Human ELISA Kit)

http://www.abcam.com/index...

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Thank you for contacting us.

This kit has not been optimised for use with tissue lysates but should be suitable for that purpose.

For the sample preparation, please note that there should not be more than 0.1% SDS present in the bu...

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Thank you for contacting us.  This kit has not been optimized for use with tissue lysates but should be suitable for that purpose.  A RIPA buffer should be fine as long as it meets the lysis buffer specifications below.  Please note that there should n...

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Abreview for Osteopontin Mouse ELISA Kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews

Sample: Mouse Tissue sections (Lung homogenate)
Specification: Lung homogenate
Amount of Sample: 10 µg
Type of Kit Used: ELISA
Positive control: Recombinant mouse OPN of known concentration.
Did you use the Abcam Kit protocol?: Yes
Username

Abcam user community

Verified customer

投稿 May 13 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"