Mouse monoclonal [SB108a] Anti-Rhesus monkey IgG heavy chain (HRP) preadsorbed (ab99707)

製品の概要

  • 製品名
    Mouse monoclonal [SB108a] Anti-Rhesus monkey IgG heavy chain (HRP) preadsorbed
    IgG 二次抗体 製品一覧
  • 由来種
    Mouse
  • ターゲット生物種
    Rhesus monkey
  • 特異性
    ab99707 reacts with Rhesus and Cynomolgous Monkey IgG heavy chain as demonstrated by ELISA.
  • アプリケーション
    適用あり: ELISAmore details
  • 吸着処理血清

    Cat, Chicken, Cow, Donkey, Goat, Guinea pig, Horse, Human, Mouse, Pig, Rabbit, Rat more details
  • 免疫原

    Rhesus IgG

  • 標識
    HRP

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    Preservative: None
    Constituents: 50% PBS, 50% Glycerol, pH 7.4
  • Concentration information loading...
  • 精製度
    Protein G purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    SB108a
  • アイソタイプ
    IgG1
  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2018 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab99707 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/1000 - 1/2000. Also suitable for fluorescent ELISA.

参考文献

ab99707 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
ELISA
We wanted to test the mouse monoclonal SB108a Anti-Rhesus monkey IgG heavy chain (HRP) preadsorbed (ab99707) to test its reactivity with IgG from Aotus nancymaae, a new world primate. The plan is to perform ELISA using this as the secondary antibody to detect anti-human IgG in the Aotus serum. Because the capture reagent is human IgG, we need this antibody to be minimally reactive with human IgG, while being reactive to the Aotus anti-human IgG. Aotus and rhesus IgGs share high levels of sequence homology.
We performed a direct ELISA with the mouse monoclonal SB108a Anti-Rhesus monkey IgG heavy chain (HRP) preadsorbed (ab99707) coating the plate with either Rhesus macaquesera, Aotus nancymaae sera and human sera. As expected, there was a detectable signal by ELISA for the rheusus sera-coated wells and not for the human sera-coated wells. Unfortunately for our intended purposes, there was no cross-reactivity with the Aotus nancymaae sera.
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投稿 Jan 31 2018

To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species: Cat, Chicken, Cow, Donkey, Goat, Guinea pig, Horse, Human, Mouse, Pig, Rabbit, Rat

Pre-adsorption (also referred to as cros...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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