Mouse monoclonal HP-6017 Anti-Human IgG Fc (FITC) (ab49867)

製品の概要

  • 製品名Mouse monoclonal HP-6017 Anti-Human IgG Fc (FITC)
    IgG 二次抗体 製品一覧
  • ターゲット生物種Human
  • 特異性This antibody reacts specifically with IgG (Fc fragment), all subclasses. The antibody does not react with IgA, IgM, purified light chains or the Fab fragment of IgG. This clone has been evaluated for specificity using a wide range of immunological techniques in the IUIS/WHO collaborative study and has been adopted by IUIS/WHO study as a standard reagent.
  • アプリケーション適用あり: Dot blotmore details
  • 免疫原

    Human IgG coupled to polyaminostyrene beads.

  • 標識FITC. Ex: 493nm, Em: 528nm

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーPreservative: 15mM Sodium Azide
    Constituents: 1% BSA (inactivated), 0.01M PBS, pH 8.0
  • Concentration information loading...
  • 精製度IgG fraction
  • 特記事項(標識)The immunoglobulin fraction of the ascites fluid was conjugated to fluorescein isothiocyanate (FITC) and then further purified to remove unconjugated FITC. F/P Molar Ratio: 3 to 5.
  • ポリ/モノモノクローナル
  • クローン名HP-6017
  • アイソタイプIgG2a
  • 特記事項

    Although the antibody site is located in the terminal end of the human IgG molecule (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor (autoantibodies directed to Fc), passage through placental membrane and staphylococcus protein A binding. A certain population of the lymphocytes posseses the "Fc receptor", therefore specific immunoreagents targeted only for the Fc fragment should prove useful.

    F-DIBA (Fluorescent Dot Immunobinding Assay): Minimum dilution of 1/16 for the conjugate on a 4-8µg dot of Human IgG (Fc fragment). F-IFMA (Particle Immunofluorescent Assay): Minimum dilution of 1/16 for the conjugate on 50µl suspension of Human IgG Agarose coated with approximately 20mg Human IgG.

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab49867 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Dot blot 1/16.

Mouse monoclonal HP-6017 Anti-Human IgG Fc (FITC) (ab49867) 使用論文

ab49867 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"