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Human IgG coupled to polyaminostyrene beads.
Although the antibody site is located in the terminal end of the human IgG molecule (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor (autoantibodies directed to Fc), passage through placental membrane and staphylococcus protein A binding. A certain population of the lymphocytes posseses the "Fc receptor", therefore specific immunoreagents targeted only for the Fc fragment should prove useful.
F-DIBA (Fluorescent Dot Immunobinding Assay): Minimum dilution of 1/16 for the conjugate on a 4-8µg dot of Human IgG (Fc fragment). F-IFMA (Particle Immunofluorescent Assay): Minimum dilution of 1/16 for the conjugate on 50µl suspension of Human IgG Agarose coated with approximately 20mg Human IgG.
Our Abpromise guarantee covers the use of ab49867 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab49867 has not yet been referenced specifically in any publications.