This antibody reacts specifically with IgG (Fc fragment), all subclasses. The antibody does not react with IgA, IgM, purified light chains or the Fab fragment of IgG. This clone has been evaluated for specificity using a wide range of immunological techniques in the IUIS/WHO collaborative study and has been adopted by IUIS/WHO study as a standard reagent.
The immunoglobulin fraction of the ascites fluid was conjugated to fluorescein isothiocyanate (FITC) and then further purified to remove unconjugated FITC.
F/P Molar Ratio: 3 to 5.
Although the antibody site is located in the terminal end of the human IgG molecule (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor (autoantibodies directed to Fc), passage through placental membrane and staphylococcus protein A binding. A certain population of the lymphocytes posseses the "Fc receptor", therefore specific immunoreagents targeted only for the Fc fragment should prove useful.
F-DIBA (Fluorescent Dot Immunobinding Assay): Minimum dilution of 1/16 for the conjugate on a 4-8µg dot of Human IgG (Fc fragment). F-IFMA (Particle Immunofluorescent Assay): Minimum dilution of 1/16 for the conjugate on 50µl suspension of Human IgG Agarose coated with approximately 20mg Human IgG.