Mouse monoclonal HP-6017 Anti-Human IgG Fc (FITC) (ab49867)

製品の概要

  • 製品名
    Mouse monoclonal HP-6017 Anti-Human IgG Fc (FITC)
    IgG 二次抗体 製品一覧
  • 由来種
    Mouse
  • ターゲット生物種
    Human
  • アプリケーション
    適用あり: Dot blotmore details
  • 免疫原

    Human IgG coupled to polyaminostyrene beads.

  • 標識
    FITC. Ex: 493nm, Em: 528nm

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    Preservative: 15mM Sodium Azide
    Constituents: 1% BSA (inactivated), 0.01M PBS, pH 8.0
  • Concentration information loading...
  • 精製度
    IgG fraction
  • 特記事項(精製)
    Purified Immunoglobulin
  • 特記事項(標識)
    The immunoglobulin fraction of the ascites fluid was conjugated to fluorescein isothiocyanate (FITC) and then further purified to remove unconjugated FITC. F/P Molar Ratio: 3 to 5.
  • ポリ/モノ
    モノクローナル
  • クローン名
    HP-6017
  • アイソタイプ
    IgG2a
  • 特記事項

    Although the antibody site is located in the terminal end of the human IgG molecule (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor (autoantibodies directed to Fc), passage through placental membrane and staphylococcus protein A binding. A certain population of the lymphocytes posseses the "Fc receptor", therefore specific immunoreagents targeted only for the Fc fragment should prove useful.

    F-DIBA (Fluorescent Dot Immunobinding Assay): Minimum dilution of 1/16 for the conjugate on a 4-8µg dot of Human IgG (Fc fragment). F-IFMA (Particle Immunofluorescent Assay): Minimum dilution of 1/16 for the conjugate on 50µl suspension of Human IgG Agarose coated with approximately 20mg Human IgG.

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab49867 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Dot blot 1/16.

参考文献

This product has been referenced in:
  • Zhou Q  et al. Ultrasound combined with targeted cationic microbubble-mediated angiogenesis gene transfection improves ischemic heart function. Exp Ther Med 13:2293-2303 (2017). Read more (PubMed: 28565841) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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