Flow Cytometry - Mouse monoclonal [2C 8F1] Secondary Antibody to Rat IgG2c - ?Fc (FITC) (ab99677)
BALB/c splenocytes were incubated with Rat IgG2a isotype, Rat anti-Mouse CD22 (2D6, Rat IgG1), Rat anti-Mouse CD8 (53-6.7, Rat IgG2a), Rat anti-Mouse CD45(LCA) (I3/2.3, Rat IgG2b), Rat anti-Mouse CD24 (30-F1, Rat IgG2c), and Rat anti-Mouse CD45RC (GL24, Rat IgM). After washing, the cells were stained with ab99677 at 0.3µg/106. Small lymphocytes were gated and analyzed.
Flow Cytometry - Mouse monoclonal [2C 8F1] Secondary Antibody to Rat IgG2c - gamma chain (FITC) (ab99677)
Top: (Negative control) ab99677 being used against BALB/c spleenocytes at 1 µg/106 cells.
Bottom: ab99677 staining BALB/c spleenocytes incubated with rat anti-mouse CD24 (IgG2c isotype) at 1 µg/106 cells.