The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use 1µg for 106 cells. ab106163-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Mouse monoclonal 2A 8F4 Anti-Rat IgG2a heavy chain (FITC) 画像
Flow Cytometry - Mouse monoclonal [2A 8F4] Secondary Antibody to Rat IgG2a - heavy chain (FITC) (ab99665)
Top: (Negative control) ab99665 being used against BALB/c spleenocytes at 1 µg/106 cells.
Bottom: ab99665 staining BALB/c spleenocytes incubated with rat anti-mouse CD8 (IgG2a isotype) at 1 µg/106 cells.