製品の概要

  • 製品名Mouse MIP2 ELISA Kit (CXCL2)
    CXCL2 キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 8 pg/ml
  • 検出範囲
    4.1 pg/ml - 1000 pg/ml
  • 添加回収試験

    90 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 94.58 86% - 104%
    Serum 88.58 83% - 99%
    Plasma 87.94 82% - 98%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Mouse
  • 製品の概要

    Abcam’s MIP2 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MIP2 in serum, plasma and cell culture supernatants. (Mouse MIP2 concentration is pretty low in normal serum/plasma, it may not be detected in this assay).

    This assay employs an antibody specific for mouse MIP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and MIP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MIP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity.
  • 配列類似性Belongs to the intercrine alpha (chemokine CxC) family.
  • 翻訳後修飾The N-terminal processed form GRO-beta(5-73) is produced by proteolytic cleavage after secretion from bone marrow stromal cells.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • C-X-C motif chemokine 2
    • Chemokine (C-X-C motif) ligand 2
    • CINC-2a
    • Cxcl2
    • CXCL2_HUMAN
    • Gro beta
    • Gro-beta
    • GRO-beta(5-73)
    • GRO-beta-T
    • GRO2
    • GRO2 oncogene
    • GROb
    • Growth-regulated protein beta
    • Hematopoietic synergistic factor
    • HSF
    • Macrophage inflammatory protein 2-alpha
    • Melanoma growth stimulatory activity beta
    • MGSA beta
    • MGSA-b
    • MIP 2
    • MIP 2a
    • MIP2
    • MIP2-alpha
    • MIP2A
    • SB-251353
    • SCYB2
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100727 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Mouse MIP2 ELISA Kit (CXCL2) 画像

  • Representative standard curve using ab100727

  • Representative standard curve using ab100727

プロトコール

Mouse MIP2 ELISA Kit (CXCL2) (ab100727) 使用論文

ab100727 has not yet been referenced specifically in any publications.

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