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|Cell culture supernatant||111||105% - 117%|
|Serum||101||94% - 110%|
|Heparin Plasma||81||76% - 89%|
|EDTA Plasma||90||87% - 93%|
|Citrate Plasma||81||77% - 84%|
MIP1b in vitro SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of MIP1b protein in mouse serum, plasma and cell culture supernatants.
The SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
MIP1b (CCL4) is a 7.8 kDa protein member of the CC subfamily of chemokines. MIP1b is induced in a variety of cells, including mast cells, CD8+ T cells, intra-epithelial gamma-delta T cells, endothelial cells, and macrophages. MIP1b is involved in the recruitment of T cells to lymph nodes and can enhance macrophage effector functions.
|内容||1 x 96 tests|
|10X Wash Buffer PT (ab206977)||1 x 20ml|
|ab221825 - Antibody Diluent 5BI||1 x 6ml|
|Mouse MIP1b Capture Antibody 10X||1 x 600µl|
|Mouse MIP1b Detector Antibody 10X||1 x 600µl|
|Mouse MIP1b Lyophilized Recombinant Protein||2 vials|
|Plate Seals||1 unit|
|SimpleStep Pre-Coated 96-Well Microplate (ab206978)||1 unit|
|Sample Diluent NS||1 x 50ml|
|Stop Solution||1 x 12ml|
|TMB Substrate||1 x 12ml|
Our Abpromise guarantee covers the use of ab215115 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Standard curve comparison between mouse MIP1b SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 0.5-fold increase in sensitivity.
Background-subtracted data values (mean +/- SD) are graphed.
The concentrations of MIP1b were measured in duplicates, interpolated from the MIP1b standard curves and corrected for sample dilution. Undiluted samples are as follows: RAW264.7 (+LPS, +IFNg) supernatant 75%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MIP1b concentration was determined to be 441 pg/mL.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MIP1b concentration was determined to be 89 pg/m/l in serum, 26 pg/mL in plasma (citrate), 28 pg/mL in plasma (heparin), 31 pg/mL in plasma (EDTA).
ab215115 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"