Mouse liver tissue lysate - total protein (14 days) (ab7265)

製品の概要

  • 製品名Mouse liver tissue lysate - total protein (14 days)
    Liver ライゼート 製品一覧
  • 製品の詳細
    Mouse liver total protein lysate
  • 特記事項

    Source: Outbred NIH-Swiss Albino mice

    The lysate was prepared by homogenization in modified RIPA buffer (50 mM Tris -HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1 mM sodium ethylenediaminetetraacetate, 1 mM phenylmethylsulfonyl flouride, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris -HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

  • アプリケーション適用あり: WBmore details

法規制情報

製品の特性

    アプリケーション

    Our Abpromise guarantee covers the use of ab7265 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    WB Use at an assay dependent concentration. Mouse liver tissue lysate is ready to load on SDS-PAGE for Western blotting. It is recommended to load 10 µg to 20 µg per lane for mini gel.

    Mouse liver tissue lysate - total protein (14 days) (ab7265) 使用論文

    ab7265 has not yet been referenced specifically in any publications.

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