Mouse liver tissue lysate - mitochondrial extract (ab110349)

製品の概要

  • 製品名
    Mouse liver tissue lysate - mitochondrial extract
    Mouse Liver ライゼート 製品一覧
  • 特記事項

    Product was previously marketed under the MitoSciences sub-brand.

    2 mg of mouse liver mitochondria in 400ul of iso-osmotic buffer. This sample can act as a positive control in immunocapture applications and Blue Native PAGE.
    1 mg solubilized mitochondria for Immunocapture experiments. For the Immunocapture of OXPHOS complexes it is recommended that 180 µl of the mitochondrial membranes supplied be solubilized by the addition of 20 µl of 10% lauryl maltoside. Insoluble material is removed by centrifugation at 72,000g before immunocapture of complexes from the supernatant.

  • アプリケーション
    適用あり: SDS-PAGE, WBmore details

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • バッファー
    Constituents: 0.02% PMSF, 0.16% Tris, 0.89% EDTA, 8.56% Sucrose
  • Concentration information loading...
  • ライゼート 備考
    2 mg of mouse liver mitochondria in 400ul of iso-osmotic buffer. This sample can act as a positive control in immunocapture applications and Blue Native PAGE.
    1 mg solubilized mitochondria for Immunocapture experiments. For the Immunocapture of OXPHOS complexes it is recommended that 180 µl of the mitochondrial membranes supplied be solubilized by the addition of 20 µl of 10% lauryl maltoside. Insoluble material is removed by centrifugation at 72,000g before immunocapture of complexes from the supernatant.

アプリケーション

Our Abpromise guarantee covers the use of ab110349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
SDS-PAGE Use at an assay dependent dilution.
WB Use at an assay dependent dilution.

参考文献

ab110349 has not yet been referenced specifically in any publications.

レビューと Q&A

Thank you for your phone call yesterday and for your patience while I have been in touch with the lab scientists at MitoSciences. Following is their reply to your questions: "These mitochondria were prepared by our mitochondrial isolation kit: http://w...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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