Mouse Leptin ELISA Kit (ab100718)
製品の概要
-
製品名Mouse Leptin ELISA Kit
Leptin キット 製品一覧 -
検出方法Colorimetric
-
サンプルの種類Cell culture supernatant, Serum, Plasma
-
アッセイタイプSandwich (quantitative)
-
検出感度< 4 pg/ml
-
検出範囲4.1 pg/ml - 1000 pg/ml
-
添加回収試験
94 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 94.47 83% - 103% Serum 93.29 82% - 102% Plasma 95.38 84% - 104% -
ステップMultiple steps standard assay
-
種交差性交差種: Mouse
-
製品の概要
Abcam’s Leptin Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Leptin in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Mouse Leptin coated on a 96- well plate. Standards and samples are pipetted into the wells and Leptin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Leptin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Leptin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
-
アプリケーション適用あり: Sandwich ELISAmore details
-
試験プラットフォームMicroplate
製品の特性
-
保存方法Store at -20°C. Please refer to protocols.
-
内容 1 x 96 tests 120X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Mouse Leptin (lyophilized) 2 vials Leptin Microplate (12 strips x 8 wells) 1 unit Recombinant Mouse Leptin Standard 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
-
機能May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
-
関連疾患Defects in LEP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
-
配列類似性Belongs to the leptin family.
-
細胞内局在Secreted.
- Information by UniProt
-
別名
- FLJ94114
- LEP
- LEP_HUMAN
see all -
参照データベース
- Entrez Gene: 16846 Mouse
- SwissProt: P41160 Mouse
- Unigene: 277072 Mouse
アプリケーション
Our Abpromise guarantee covers the use of ab100718 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
画像
-
ab100718 Leptin Mouse ELISA KitMs Leptin measured in biological fluids showing quantity (pg) per mL of tested sample
-
ab100718 Leptin Mouse ELISA KitMs Leptin measured in cell lysates showing quantity (pg) per mg protein
-
Typical Standard CurveRepresentative Standard Curve using ab100718
-
Typical Standard CurveRepresentative Standard Curve using ab100718
プロトコール
参考文献
This product has been referenced in:
- Losada-Barragán M et al. Protein malnutrition promotes dysregulation of molecules involved in T cell migration in the thymus of mice infected with Leishmania infantum. Sci Rep 7:45991 (2017). ELISA ; Mouse . Read more (PubMed: 28397794) »
- Nuñez-Durán E et al. Protein kinase STK25 aggravates the severity of non-alcoholic fatty pancreas disease in mice. J Endocrinol 234:15-27 (2017). ELISA . Read more (PubMed: 28442507) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"