94
%
サンプルの種類 | 平均 % | 測定範囲 |
---|---|---|
Cell culture supernatant | 94.47 | 83% - 103% |
Serum | 93.29 | 82% - 102% |
Plasma | 95.38 | 84% - 104% |
Abcam’s Leptin Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Leptin in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Mouse Leptin coated on a 96- well plate. Standards and samples are pipetted into the wells and Leptin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Leptin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Leptin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
内容 | 1 x 96 tests |
---|---|
120X HRP-Streptavidin Concentrate | 1 x 200µl |
20X Wash Buffer Concentrate | 1 x 25ml |
5X Assay Diluent B | 1 x 15ml |
Assay Diluent A | 1 x 30ml |
Biotinylated anti-Mouse Leptin (lyophilized) | 2 vials |
Leptin Microplate (12 strips x 8 wells) | 1 unit |
Recombinant Mouse Leptin Standard | 2 vials |
Stop Solution | 1 x 8ml |
TMB One-Step Substrate Reagent | 1 x 12ml |
Our Abpromise guarantee covers the use of ab100718 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Sandwich ELISA | Use at an assay dependent concentration. |
Ms Leptin measured in biological fluids showing quantity (pg) per mL of tested sample
Ms Leptin measured in cell lysates showing quantity (pg) per mg protein
Representative Standard Curve using ab100718
Representative Standard Curve using ab100718
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"