製品の概要

  • 製品名
    Mouse Laminin ELISA Kit
    Laminin キット 製品一覧
  • 検出方法
    Colorimetric
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    < 10 pg/ml
  • 検出範囲
    156 pg/ml - 10000 pg/ml
  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Mouse
  • 製品の概要

    Abcam’s mouse Laminin in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of mouse Laminin in cell culture supernatants, serum and plasma (heparin, EDTA).

    A polyclonal antibody from rabbit specific for Laminin has been precoated onto 96-well plates. Standards(from murine sarcoma basement membrane) and test samples are added to the wells, a biotinylated detection polyclonal antibody from rabbit specific for Laminin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Laminin amount of sample captured in plate.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

法規制情報

製品の特性

  • 保存方法
    Store at -20°C. Please refer to protocols.
  • 内容 1 x 96 tests
    ABC Diluent Buffer 1 x 12ml
    Antibody Diluent Buffer 1 x 12ml
    Anti-mouse Laminin Antibody Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti-mouse Laminin antibody 1 x 130µl
    Lyophilized Recombinant mouse Laminin standard 2 x 10ng
    Plate Seal 1 x 4 units
    Sample Diluent Buffer 1 x 30ml
    TMB Color Developing Agent 1 x 10ml
    TMB Stop Solution 1 x 10ml
  • 研究分野
  • 機能
    Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
  • 関連疾患
    Defects in LAMB2 are the cause of Pierson syndrome (PIERSS) [MIM:609049]; also known as microcoria-congenital nephrotic syndrome. Pierson syndrome is characterized by nephrotic syndrome with neonatal onset, diffuse mesangial sclerosis and eye abnormalities with microcoria as the leading clinical feature. Death usually occurs within the first weeks of life. Disease severity depends on the mutation type: nontruncating LAMB2 mutations may display variable phenotypes ranging from a milder variant of Pierson syndrome to isolated congenital nephrotic syndrome.
    Defects in LAMB2 are a cause of congenital nephrotic syndrome (CONPHS) [MIM:609049]. Congenital nephrotic syndrome constitutes a heterogeneous group of conditions having in common the disruption of normal glomerular permselectivity. Congenital nephrotic syndrome due to LAMB2 mutations may be associated with ocular abnormalities.
  • 配列類似性
    Contains 13 laminin EGF-like domains.
    Contains 1 laminin IV type B domain.
    Contains 1 laminin N-terminal domain.
  • ドメイン
    The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
    Domains VI and IV are globular.
  • 細胞内局在
    Secreted > extracellular space > extracellular matrix > basement membrane. S-laminin is concentrated in the synaptic cleft of the neuromuscular junction.
  • Information by UniProt
  • 別名
    • Lamb2
    • LAMB2_HUMAN
    • LAMC1
    • LAMC2
    • LAMC3
    • laminin A
    • Laminin B
    • Laminin B1s chain
    • Laminin gamma 1
    • Laminin gamma 2
    • Laminin gamma 3
    • Laminin subunit beta-2
    • Laminin-11 subunit beta
    • Laminin-14 subunit beta
    • Laminin-15 subunit beta
    • Laminin-3 subunit beta
    • Laminin-4 subunit beta
    • Laminin-7 subunit beta
    • Laminin-9 subunit beta
    • OCCM
    • S-LAM beta
    • S-laminin subunit beta
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab119572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Representative Standard Curve using ab119572.

プロトコール

参考文献

ab119572 has not yet been referenced specifically in any publications.

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