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Abcam’s mouse Laminin in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of mouse Laminin in cell culture supernatants, serum and plasma (heparin, EDTA).
A polyclonal antibody from rabbit specific for Laminin has been precoated onto 96-well plates. Standards(from murine sarcoma basement membrane) and test samples are added to the wells, a biotinylated detection polyclonal antibody from rabbit specific for Laminin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Laminin amount of sample captured in plate.
|内容||1 x 96 tests|
|ABC Diluent Buffer||1 x 12ml|
|Antibody Diluent Buffer||1 x 12ml|
|Anti-mouse Laminin Antibody Microplate (12 x 8 wells)||1 unit|
|Avidin-Biotin-Peroxidase Complex (ABC)||1 x 130µl|
|Biotinylated anti-mouse Laminin antibody||1 x 130µl|
|Lyophilized Recombinant mouse Laminin standard||2 x 10ng|
|Plate Seal||1 x 4 units|
|Sample Diluent Buffer||1 x 30ml|
|TMB Color Developing Agent||1 x 10ml|
|TMB Stop Solution||1 x 10ml|
Our Abpromise guarantee covers the use of ab119572 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab119572 has not yet been referenced specifically in any publications.