Mouse kidney tissue lysate - total protein (0 days) (ab7261)

製品の概要

• 製品名Mouse kidney tissue lysate - total protein (0 days)
  Kidney ライゼート 製品一覧
• 製品の詳細
  Mouse kidney total protein lysate
• 特記事項

  Outbred NIH-Swiss Albino mice (0 days old).

• アプリケーション適用あり: WBmore details

製品の特性

• 製品の状態Liquid
• 保存方法Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
• バッファーPreservative: None
  Constituents: 5% Beta mercaptoethanol, 12.5% Glycerol, 1% SDS, 1% Triton-X-100, 1% Sodium Deoxycholate, 0.01% Bromophenol blue, 5µg/ml Leupeptin, 5µg/ml Aprotinin, 50mM Tris HCl, 150mM Sodium chloride, 1mM EDTA, 1mM PMSF, pH 6.8
• Concentration information loading...
• ライゼート 備考The mouse kidney tissue was collected from zero (0) day-old mice. The lysate was prepared by homogenization in modified RIPA buffer (50 mM Tris -HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1 mM sodium ethylenediaminetetraacetate, 1 mM phenylmethylsulfonyl flouride, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris -HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
• 研究分野
  • Kits/ Lysates/ Other
  • Lysates
  • Tissue Lysates
  • Mouse
  • Genito-Urinary

  • Kits/ Lysates/ Other
  • Lysates
  • Whole Cell Lysates
  • Mouse
  • Genito-Urinary