Mouse kidney tissue lysate (14 days) (ab7262)

製品の概要

  • 製品名Mouse kidney tissue lysate (14 days)
    Kidney ライゼート 製品一覧
  • 製品の詳細
    Mouse kidney lysate
  • 特記事項Source: Outbred NIH-Swiss Albino mice (14 days old)


    The mouse kidney tissue was collected from fourteen (14) day-old mice. The lysate was prepared by homogenization in modified RIPA buffer (50 mM Tris -HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1 mM sodium ethylenediaminetetraacetate, 1 mM phenylmethylsulfonyl flouride, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris -HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

  • アプリケーション適用あり: WBmore details

法規制情報

製品の特性

    アプリケーション

    Our Abpromise guarantee covers the use of ab7262 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    WB Use at an assay dependent concentration. Mouse kidney tissue lysate is ready to load on SDS-PAGE for Western blotting. It is recommended to load 10 µg to 20 µg per lane for mini gel.

    Mouse kidney tissue lysate (14 days) (ab7262) 使用論文

    ab7262 has not yet been referenced specifically in any publications.

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