製品の概要

  • 製品名Mouse IP10 ELISA Kit
    IP10 キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 15 pg/ml
  • 検出範囲
    8.2 pg/ml - 2000 pg/ml
  • 添加回収試験

    97 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 98.86 88% - 107%
    Serum 97.68 86% - 106%
    Plasma 96.42 85% - 104%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Mouse
  • 製品の概要

    Abcam’s IP10 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IP10 in serum, plasma and cell culture supernatants.


    This assay employs an antibody specific for mouse IP10 coated on a 96- well plate. Standards and samples are pipetted into the wells and IP10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IP10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IP10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Save time with a 90-minute ELISA protocol. Try Mouse IP-10 ELISA Kit (CXCL10) (ab214563) from our SimpleStep ELISA® range.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Chemotactic for monocytes and T-lymphocytes. Binds to CXCR3.
  • 配列類似性Belongs to the intercrine alpha (chemokine CxC) family.
  • 翻訳後修飾CXCL10(1-73) is produced by proteolytic cleavage after secretion from keratinocytes.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • Interferon gamma induced factor MOB1, mouse, homolog of
    • Interferon gamma induced protein 10
    • 10 kDa interferon gamma induced protein
    • 10 kDa interferon gamma-induced protein
    • C X C motif chemokine 10
    • C7
    • Chemokine (C X C motif) ligand 10
    • Chemokine CXC motif ligand 10
    • Crg 2
    • CRG2
    • CXCL10
    • CXCL10(1-73)
    • CXL10_HUMAN
    • Gamma IP10
    • Gamma-IP10
    • gIP 10
    • GIP10
    • IFI10
    • INP 10
    • INP10
    • Interferon activated gene 10
    • Interferon gamma induced cell line
    • Interferon inducible cytokine IP 10
    • Interferon inducible cytokine IP10
    • IP 10
    • IP-10
    • Mob 1
    • MOB1
    • Protein 10 from interferon (gamma) induced cell line
    • SCYB10
    • Small inducible cytokine B10
    • Small inducible cytokine B10 precursor
    • Small inducible cytokine subfamily B (Cys X Cys) member 10
    • Small inducible cytokine subfamily B CXC member 10
    • Small inducible cytokine subfamily B, member 10
    • Small-inducible cytokine B10
    see all
  • 参照データベース

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab100675 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Mouse IP10 ELISA Kit 画像

  • Representative Standard Curve using ab100675

  • Representative Standard Curve using ab100675

プロトコール

Mouse IP10 ELISA Kit (ab100675) 使用論文

ab100675 has not yet been referenced specifically in any publications.

Product Wall

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Thank you for your inquiry. Sorry for the delayed reply. Item F (Detection Antibody CRG-2) of ELM-CRG2-001 comes as a very concentrated, small volume liquid. Usually the volume is very small, ~2ul, so it may be difficult to see. This is why we ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"