Mouse Interferon gamma ELISPOT Kit (ab64029)

製品の概要

  • 製品名Mouse Interferon gamma ELISPOT Kit
    Interferon gamma キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Cell culture extracts
  • アッセイタイプSandwich (qualitative)
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Mouse
  • 製品の概要

    The ELISPOT assay is designed to enumerate cytokine producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in-vitro manipulations allowing cytokine production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. Elispot assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, cancerology, infectious diseases, autoimmune diseases and transplantation.


    Abcam Elispot assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.


    Principle


    After cell stimulation, locally produced cytokines are captured by a specific monoclonal antibody. After cell lysis, trapped cytokine molecules are revealed by a secondary biotinylated detection antibody, which is in turn recognised by streptavidin conjugated to alkaline phosphatase. PVDF-bottomed-well plates are then incubated with BCIP/NBT substrate. Colored "purple" spots indicate cytokine production by individual cells.


     


    Recognizes natural murine Interferon gamma.

  • アプリケーション適用あり: ELISpotmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 関連性Interferon gamma is a dimerized soluble cytokine that is the only member of the type II class of interferons. In contrast to interferon alpha and interferon beta which can be expressed by all cells, Interferon gamma is secreted by T lymphocytes and NK cells only. Also known as immune interferon, Interferon gamma is the only Type II interferon. It is serologically distinct from Type I interferons and it is acid-labile, while the type I variants are acid-stable. Interferon gamma has antiviral, immunoregulatory, and anti-tumour properties. It alters transcription in up to 30 genes producing a variety of physiological and cellular responses. Activation by Interferon gamma is achieved by its interaction with a heterodimeric receptor consisting of IFNGR1 & IFNGR2 (interferon gamma receptors). Interferon gamma binding to the receptor activates the JAK-STAT pathway. In addition, Interferon gamma activates APCs and promotes Th1 differentiation by upregulating the transcription factor T-bet. Interferon gamma is the hallmark cytokine of Th1 cells (Th2 cells produce IL-4). NK cells and CD8+ cytotoxic T cells also produce Interferon gamma. Interferon gamma suppresses osteoclast formation by rapidly degrading the RANK adaptor protein TRAF6 in the RANK-RANKL signaling pathway, which otherwise stimulates the production of NF?B.
  • 細胞内局在Secreted
  • 別名
    • IFG
    • IFI
    • IFN gamma
    • IFNG
    • Immune interferon
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab64029 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISpot Use at an assay dependent dilution.

プロトコール

Mouse Interferon gamma ELISPOT Kit (ab64029) 使用論文

ab64029 has not yet been referenced specifically in any publications.

Product Wall

This kit ships with whole 96 wells plates, not 12, 8-well strips.



Unfortunately, this kit is a ELISpot assay dedicated to detect IFNg secreting cells and not appropriate for serum.

This product will work for your customer's samples:

http://www.abcam.com/index.html?datasheet=46081 (or use ...

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Thank you for contacting us.

The collaborator lab who devolved this kit have suggested using the following stimulation protocol :
Pre-stimulation of splenocytes by ConA (0.5µg/ml) + murine IL-2 (2ng/ml) 24h (or 48h), then addition of...

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Thank you for your email.

Peter is in feedback department. They do not deal with technical inquiries. I have been assigned your case and I will be following up now on. I have checked your previous contacts with my colleagues it seems you have ...

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Thank you for calling us.

I am very sorry that we had so many problems sending you the emails. I am happy that it seems to work now.

I apologize also that our phone call got cut. Please do not hesitate to call back.

As we...

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Thank youfor your phone call yesterday.

I am very sorry for the error on our datasheet in regards to the plate format. I have investigated this issue with my colleagues, and indeed, the kit ab64029 comes onlywith one 96 well plate. we have up...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"