Mouse IL-7 ELISA Kit (Interleukin-7) (ab100714)

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製品の概要

  • 製品名
    Mouse IL-7 ELISA Kit (Interleukin-7)
    IL7 キット 製品一覧
  • 検出方法
    Colorimetric
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    < 10 pg/ml
  • 検出範囲
    8.23 pg/ml - 6000 pg/ml
  • 添加回収試験

    93 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 94.58 85% - 105%
    Serum 91.59 82% - 103%
    Plasma 92.66 83% - 104%

  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Mouse
  • 製品の概要

    Abcam’s IL-7 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-7 in serum, plasma and cell culture supernates.

    This assay employs an antibody specific for mouse IL-7 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-7 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-7 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-7 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

製品の特性

  • 保存方法
    Store at -20°C. Please refer to protocols.
  • 内容 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    440X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-mouse IL-7 2 vials
    IL-7 Microplate (12 x 8 wells) 1 unit
    Recombinant mouse IL-7 Standard 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • 研究分野
  • 関連性
    IL7 is a cytokine important for B and T cell development. It and the hepatocyte growth factor form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. IL7 is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. It can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. Knockout studies in mice suggested that IL7 plays an essential role in lymphoid cell survival.
  • 細胞内局在
    Secreted
  • 別名
    • IL 7
    • IL7 nirs variant 6
    • Interleukin 7
    • Interleukin7
  • 参照データベース

    アプリケーション

    Our Abpromise guarantee covers the use of ab100714 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    Sandwich ELISA Use at an assay dependent concentration.

    画像

    • Typical standard curve
      Typical standard curve

      Representative standard curve using ab100714

    • Typical standard curve
      Typical standard curve

      Representative standard curve using ab100714

    プロトコール

    参考文献

    ab100714 has not yet been referenced specifically in any publications.

    ab100714 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

    レビューを送る 質問を送る

    1 Review or Q&A

    10
    Votes: Highest First

    Will this antibody react with both the membrane bound and soluble protein? Can it distinguish between the two?

    Thank you for contacting Abcam regarding ab100714.


    The IL7 Mouse ELISA Kit will detect soluble IL-7. As this kit was not validated with cell or tissue lysate samples, we do not know if the kit will also detect IL-7 bound to its membrane...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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