93
%
サンプルの種類 | 平均 % | 測定範囲 |
---|---|---|
Cell culture supernatant | 94.58 | 85% - 105% |
Serum | 91.59 | 82% - 103% |
Plasma | 92.66 | 83% - 104% |
Abcam’s IL-7 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-7 in serum, plasma and cell culture supernates.
This assay employs an antibody specific for mouse IL-7 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-7 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-7 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-7 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
内容 | 1 x 96 tests |
---|---|
20X Wash Buffer Concentrate | 1 x 25ml |
440X HRP-Streptavidin Concentrate | 1 x 200µl |
5X Assay Diluent B | 1 x 15ml |
Assay Diluent A | 1 x 30ml |
Biotinylated anti-mouse IL-7 | 2 vials |
IL-7 Microplate (12 x 8 wells) | 1 unit |
Recombinant mouse IL-7 Standard | 2 vials |
Stop Solution | 1 x 8ml |
TMB One-Step Substrate Reagent | 1 x 12ml |
Our Abpromise guarantee covers the use of ab100714 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Sandwich ELISA | Use at an assay dependent concentration. |
ab100714 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"