製品の概要

  • 製品名
    Mouse IL-4 ELISA Kit (Interleukin-4)
    IL4 キット 製品一覧
  • 検出方法
    Colorimetric
  • サンプルの種類
    Cell culture extracts, Tissue Extracts
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    < 1 pg/ml
  • 検出範囲
    0.819 pg/ml - 200 pg/ml
  • 添加回収試験

    92 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture extracts 93.62 84% - 104%
    Tissue Extracts 91.57 82% - 103%

  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Mouse
  • 製品の概要

    Abcam’s Mouse IL-4 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-4 in cell lysates and tissue lysates.

    This assay employs an antibody specific for mouse IL-4 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

法規制情報

製品の特性

  • 保存方法
    Store at -20°C. Please refer to protocols.
  • 内容 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    2x Cell lysate buffer 1 x 5ml
    5X Assay Diluent 1 x 15ml
    5X Sample Diluent Buffer 1 x 10ml
    600X HRP-Streptavidin Concentrate 1 x 200µl
    Biotinylated anti-mouse IL-4 (lyophilized) 2 vials
    IL-4 Microplate (12 x 8 wells) 1 unit
    Recombinant mouse IL-4 Standard 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Reagent 1 x 12ml
  • 研究分野
  • 関連性
    IL4 is a pleiotropic cytokine produced by activated T cells. It is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of IL4. IL4, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. IL4, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of IL4 encoding distinct isoforms have been reported.
  • 細胞内局在
    Secreted
  • 別名
    • B cell growth factor 1
    • B cell stimulatory factor 1
    • BCGF 1
    • BCGF1
    • Binetrakin
    • BSF 1
    • BSF1
    • IL 4
    • Interleukin 4
    • Lymphocyte stimulatory factor 1
    • MGC79402
    • Pitrakinra
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100710 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Representative standard curve using ab100710

プロトコール

参考文献

This product has been referenced in:
  • Hajmohammadi M  et al. Synthesis and characterization of a novel chemically designed (Globo)3-DTPA-KLH antigen. Drug Des Devel Ther 9:217-39 (2015). Sandwich ELISA ; Mouse . Read more (PubMed: 25565775) »

See 1 Publication for this product

レビューと Q&A

I have broken down the cross-reactivity information for each kit below:


· ab100549 IL10 Human ELISA Kit
o The capture antibody did not show any cross-reactivity with Murine IL-10 from testing. Thus, this kit is not expected ...

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Thank you for your inquiry.

I can confirm that the sample diluent buffer would be the most suitable buffer to dilute the serum samples.

I suggest to do a serial dilution to find the optimal dilution.

I hope this informatio...

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Thank you for your inquiry.

I can confirm that these kit are suitable for the use with serum samples that have been frozen.

I recommend to not to mix/compare samples thathave been frozen with samples that have not been frozen unless...

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Thank you for your inquiry. I can confirm that all these kits are suitable for mouse samples: ab83362 and ab102512: These kits can be used with a variety of samples such as serum, plasma, and urine and should not be species dependent. As long as there ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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