製品の概要

  • 製品名
    Mouse IL-28 ELISA Kit (Interleukin-28)
  • 検出方法
    Colorimetric
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    < 2 pg/ml
  • 検出範囲
    2.46 pg/ml - 600 pg/ml
  • 添加回収試験

    88 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 74.3 67% - 87%
    Serum 109.8 98% - 118%
    Plasma 76.8 67% - 93%

  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Mouse
  • 製品の概要

    Abcam’s IL-28 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IL-28 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for IL-28 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-28 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse IL-28 antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-28 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab100708 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Representative standard curve using ab100708

プロトコール

参考文献

ab100708 has not yet been referenced specifically in any publications.

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