Mouse IL-23 ELISA Kit (Interleukin-23) (ab119545)

製品の概要

  • 製品名Mouse IL-23 ELISA Kit (Interleukin-23)
    IL23 キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 8.3%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 2.5%
  • テスト
    1 x 96 test
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    16 pg/ml
  • 検出範囲
    31.3 pg/ml - 2000 pg/ml
  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Serum 49 41% - 53%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Mouse
  • 製品の概要

    Abcam’s IL-23 (Interleukin-23) Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IL-23 in cell culture supernatant, serum and plasma (citrate).

    IL-23 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotin-conjugated IL-23 detection antibody then incubated at room temperature. Following washing, a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-23 captured on the plate.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

法規制情報

製品の特性

  • 機能Associates with IL12B to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to an heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
  • 組織特異性Secreted by activated dendritic and phagocytic cells and keratinocytes. Also expressed by dermal Langerhans cells (at protein level).
  • 配列類似性Belongs to the IL-6 superfamily.
  • 発生段階Expressed by newborns dendritic cells.
  • 細胞内局在Secreted. Secreted upon association with IL12B.
  • Information by UniProt
  • 別名
    • IL 23
    • IL 23A
    • IL-23 subunit alpha
    • IL-23-A
    • IL-23p19
    • Il23a
    • IL23A_HUMAN
    • IL23P19
    • Interleukin 23 alpha subunit p19
    • Interleukin-23 subunit alpha
    • Interleukin-23 subunit p19
    • JKA3 induced upon T cell activation
    • P19
    • SGRF
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab119545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Mouse IL-23 ELISA Kit (Interleukin-23) 画像

  • Representative Standard Curve using ab119545.

     

プロトコール

Mouse IL-23 ELISA Kit (Interleukin-23) (ab119545) 使用論文

ab119545 has not yet been referenced specifically in any publications.

Product Wall



Ab119545 has only been tested with culture supernatant, serum, and citrate plasma, but it may be suitable for use with untested sample types like tissue lysates.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"