Mouse IL-2 ELISA Kit (Interleukin-2) (ab46096)
製品の概要
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製品名Mouse IL-2 ELISA Kit (Interleukin-2)
IL2 キット 製品一覧 -
検出方法Colorimetric
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再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% A 10 170pg/ml 13 7.8% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% B 8 137pg/ml 8.9 6.4% -
サンプルの種類Cell culture supernatant, Serum
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アッセイタイプSandwich (quantitative)
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検出感度< 5 pg/ml
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検出範囲15.6 pg/ml - 500 pg/ml
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全工程の試験時間3h 45m
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ステップMultiple steps standard assay
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種交差性交差種: Mouse
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製品の概要
Abcam’s IL-2 (Interleukin-2) mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-2 in mouse serum, buffered solutions and cell culture medium.
A monoclonal antibody specific for IL-2 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-2 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-2 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-2 present in the samples.
This kit will recognize both endogenous and recombinant mouse IL-2.
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アプリケーション適用あり: Sandwich ELISAmore details
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試験プラットフォームMicroplate
法規制情報
製品の特性
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保存方法Store at +4°C. Please refer to protocols.
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内容 ラベル 1 x 96 tests 2 x 96 tests 10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml 200X Wash Buffer White 1 x 10ml 2 x 10ml Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml Biotinylated anti-IL-2 Red 1 x 400µl 1 x 400µl Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml HRP Diluent Red 1 x 23ml 1 x 23ml IL-2 Microplate (12 x 8 well strips) 1 unit 2 units IL-2 Standard (Lyophilized) Yellow 2 vials 4 vials Stop Reagent Black 1 x 11ml 2 x 11ml Streptavidin HRP 2 x 5µl 4 x 5µl -
研究分野
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機能Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
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関連疾患Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17.
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配列類似性Belongs to the IL-2 family.
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細胞内局在Secreted.
- Information by UniProt
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別名
- Aldesleukin
- IL 2
- IL-2
see all -
参照データベース
- Entrez Gene: 16183 Mouse
- SwissProt: P04351 Mouse
- Unigene: 14190 Mouse
アプリケーション
Our Abpromise guarantee covers the use of ab46096 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Mouse IL-2 ELISA Kit (Interleukin-2) 画像
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Typical Standard CurveRepresentative Standard Curve using ab46096.
プロトコール
Mouse IL-2 ELISA Kit (Interleukin-2) (ab46096) 使用論文
ab46096 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"