サンプル | N | 平均値 | SD | CV% |
---|---|---|---|---|
A | 10 | 170pg/ml | 13 | 7.8% |
サンプル | N | 平均値 | SD | CV% |
---|---|---|---|---|
B | 8 | 137pg/ml | 8.9 | 6.4% |
Abcam’s IL-2 (Interleukin-2) mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-2 in mouse serum, buffered solutions and cell culture medium.
A monoclonal antibody specific for IL-2 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-2 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-2 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-2 present in the samples.
This kit will recognize both endogenous and recombinant mouse IL-2.
内容 | ラベル | 1 x 96 tests | 2 x 96 tests |
---|---|---|---|
10X Standard Diluent Buffer | Black | 1 x 25ml | 1 x 25ml |
200X Wash Buffer | White | 1 x 10ml | 2 x 10ml |
Biotinylated Antibody Diluent | Red | 1 x 7.5ml | 1 x 13ml |
IL2 Biotinylated Detection antibody | 1 x 400µl | 1 x 400µl | |
Chromogen TMB Substrate Solution | 1 x 11ml | 1 x 24ml | |
HRP Diluent | Red | 1 x 23ml | 1 x 23ml |
IL-2 Microplate (12 x 8 well strips) | 1 unit | 2 units | |
Murine IL2 standard (Lyophilized) | Yellow | 2 vials | 4 vials |
Stop Reagent | Black | 1 x 11ml | 2 x 11ml |
Streptavidin-HRP | 2 x 5µl | 4 x 5µl |
Our Abpromise guarantee covers the use of ab46096 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Sandwich ELISA | Use at an assay dependent concentration. |
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"