製品の概要

  • 製品名
    Mouse IL-2 ELISA Kit (Interleukin-2)
    IL2 キット 製品一覧
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay（同時再現性）
    サンプル N 平均値 SD CV%
    A 10 170pg/ml 13 7.8%
    Inter-Assay（日差再現性）
    サンプル N 平均値 SD CV%
    B 8 137pg/ml 8.9 6.4%
  • サンプルの種類
    Cell culture supernatant, Serum
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    < 5 pg/ml
  • 検出範囲
    15.6 pg/ml - 500 pg/ml
  • 全工程の試験時間
    3h 45m
  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Mouse
  • 製品の概要

    Abcam’s IL-2 (Interleukin-2) mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-2 in mouse serum, buffered solutions and cell culture medium.

    A monoclonal antibody specific for IL-2 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-2 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-2 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-2 present in the samples.

    This kit will recognize both endogenous and recombinant mouse IL-2.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

法規制情報

医薬用外毒物

製品の特性

  • 保存方法
    Store at +4°C. Please refer to protocols.
  • 内容 ラベル 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    200X Wash Buffer White 1 x 10ml 2 x 10ml
    Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml
    IL2 Biotinylated Detection antibody 1 x 400µl 1 x 400µl
    Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
    HRP Diluent Red 1 x 23ml 1 x 23ml
    IL-2 Microplate (12 x 8 well strips) 1 unit 2 units
    Murine IL2 standard (Lyophilized) Yellow 2 vials 4 vials
    Stop Reagent Black 1 x 11ml 2 x 11ml
    Streptavidin-HRP 2 x 5µl 4 x 5µl
  • 研究分野
  • 機能
    Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
  • 関連疾患
    Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17.
  • 配列類似性
    Belongs to the IL-2 family.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 別名
    • Aldesleukin
    • IL 2
    • IL-2
    • IL2
    • IL2_HUMAN
    • Interleukin 2
    • Interleukin-2
    • Interleukin2
    • Involved in regulation of T cell clonal expansion
    • Lymphokine
    • T cell growth factor
    • T-cell growth factor
    • TCGF
    see all
  • 参照データベース

    アプリケーション

    Our Abpromise guarantee covers the use of ab46096 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    Sandwich ELISA Use at an assay dependent concentration.

    画像

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab46096.

    プロトコール

    参考文献

    This product has been referenced in:
    • Zamecnik CR  et al. Injectable Polymeric Cytokine-Binding Nanowires Are Effective Tissue-Specific Immunomodulators. ACS Nano 11:11433-11440 (2017). ELISA ; Mouse . Read more (PubMed: 29124929) »

    See 1 Publication for this product

    ab46096 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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