製品の概要

  • 製品名Mouse IL-1ra ELISA Kit (Interleukin-1ra)
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 2 pg/ml
  • 検出範囲
    2.74 pg/ml - 2000 pg/ml
  • 添加回収試験

    90 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 102 94% - 103%
    Serum 75.89 67% - 84%
    Plasma 93.69 71% - 111%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rat, Human
  • 製品の概要

    Abcam’s IL-1ra Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of IL-1ra in serum, plasma (collect plasma using EDTA and heparin as an anticoagulant. Citrate is not recommended for this assay) and cell culture supernates.

    This assay employs an antibody specific for mouse IL-1ra coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1ra present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-1ra antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1ra bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure.
  • 組織特異性The intracellular form of IL1RN is predominantly expressed in epithelial cells.
  • 関連疾患Genetic variation in IL1RN is associated with susceptibility to microvascular complications of diabetes type 4 (MVCD4) [MIM:612628]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
    Defects in IL1RN are the cause of interleukin 1 receptor antagonist deficiency (DIRA) [MIM:612852]; also known as deficiency of interleukin 1 receptor antagonist. Autoinflammatory diseases manifest inflammation without evidence of infection, high-titer autoantibodies, or autoreactive T-cells. DIRA is a rare, autosomal recessive, genetic autoinflammatory disease that results in sterile multifocal osteomyelitis (bone inflammation in multiple places), periostitis (inflammation of the membrane surrounding the bones), and pustulosis (due to skin inflammation) from birth.
  • 細胞内局在Cytoplasm and Secreted.
  • Information by UniProt
  • 別名
    • ICIL-1RA
    • IL-1ra
    • IL-1RN
    • IL1 inhibitor
    • IL1RA_HUMAN
    • IL1RN
    • Interleukin-1 receptor antagonist protein
    • IRAP
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab113348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Mouse IL-1ra ELISA Kit (Interleukin-1ra) 画像

  • Standard curve of msIL-1Ra in different diluents with background signal subtracted (duplicates; +/- SD).
  • IL-1Ra measured in mouse tissue lysates (expressed as per mg of extracted protein), with background signal subtracted (duplicates; +/- SD).
  • msIL-1Ra measured in biological fluids, background signal subtracted (duplicates +/- SD).
  • IL-1Ra detected in supernatants from RAW 246.7 control cells (C) or cells stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297) (P), or 24 hours with PMA and 1 ug x mL-1 of LPS (Sigma) (P+L) for the last 6 hours. Results shown after background signal was subtracted (duplicates +/- SD).
  • Representative Standard Curve using ab113348

  • Representative Standard Curve using ab113348

プロトコール

Mouse IL-1ra ELISA Kit (Interleukin-1ra) (ab113348) 使用論文

ab113348 has not yet been referenced specifically in any publications.

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