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Abcam’s IL-17F (Interleukin-17) Mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Mouse IL-17F (Interleukin-17)concentrations in cell culture supernatant, serum and plasma (EDTA).
IL-17F specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and then incubated at room temperature. After washing, a Biotin-conjugated anti-Human IL-17F detection antibody is added then incubated at room temperature. Following washing Streptavidin-HRP conjugate is added to each well, incubated at room temperature then again washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-17F captured on the plate.
|内容||1 x 96 tests|
|20X Assay Buffer Concentrate||1 x 5ml|
|20X Wash Buffer Concentrate||1 x 50ml|
|Adhesive Films||6 units|
|Biotin-Conjugate anti-mouse IL17F monoclonal antibody||1 x 120µl|
|Calibrator Diluent||1 x 5ml|
|Conjugate Diluent||1 x 15ml|
|Microplate coated with monoclonal antibody to Mouse IL-17F (12 x 8 wells)||1 unit|
|Mouse IL-17F Standard lyophilized (4 ng/ml upon reconstitution)||2 vials|
|Sample Diluent||1 x 12ml|
|Stop Solution (1M Phosphoric acid)||1 x 15ml|
|Streptavidin-HRP||1 x 150µl|
|TMB Substrate Solution||1 x 15ml|
Our Abpromise guarantee covers the use of ab119538 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab119538 has not yet been referenced specifically in any publications.