製品の概要

  • 製品名
    Mouse IL-17A ELISA Kit
    IL-17a キット 製品一覧
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Serum 5 4.9%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Serum 3 10.6%
  • サンプルの種類
    Cell culture supernatant, Serum, Citrate Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    0.5 pg/ml
  • 検出範囲
    6.25 pg/ml - 400 pg/ml
  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Serum 102.5 97.7% - 105.8%
    Cell culture media 91.1 82.7% - 99.7%
    Citrate Plasma 103.6 102.2% - 105.8%

  • 全工程の試験時間
    1h 30m
  • ステップ
    One step assay
  • 種交差性
    交差種: Mouse
  • 製品の概要

    IL-17A mouse in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-17A protein in mouse serum, plasma (citrate) and cell culture supernatant samples.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Sensitivity:


    Samples diluted in Sample Diluent NS – 0.5 pg/mL


    Samples diluted in Sample Diluent 25BP – 5.5 pg/mL

  • 特記事項

    IL-17A is a pro-inflammatory cytokine that is secreted by a subset of activated T cells. It is a disulfide-linked homodimer with both glycosylated and non-glycosylated forms. IL-17A induces stromal cells to produce pro-inflammatory and hematopoietic cytokines, and also enhances the surface expression of ICAM1/intracellular adhesion molecule 1 in fibroblasts.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate (12 x 8 well strips)

製品の特性

  • 保存方法
    Store at +4°C. Please refer to protocols.
  • 内容 1 x 96 tests
    10X Mouse IL-17a Capture Antibody 1 x 600µl
    10X Mouse IL-17a Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml
    Mouse IL-17a Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent 25BP 1 x 20ml
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Substrate 1 x 12ml
  • 研究分野
  • 機能
    Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of ICAM1/intracellular adhesion molecule 1 in fibroblasts.
  • 組織特異性
    Restricted to activated memory T-cells.
  • 配列類似性
    Belongs to the IL-17 family.
  • 翻訳後修飾
    Found both in glycosylated and nonglycosylated forms.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 別名
    • CTLA8
    • IL17A
    • CTLA-8
    • Cytotoxic T-lymphocyte-associated antigen 8
    • IL-17
    • IL-17A
    • IL17
    • IL17_HUMAN
    • Il17a
    • Interleukin-17A
    see all
  • 参照データベース

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab199081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Standard curve comparison between mouse IL-17A SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 9-fold increase in sensitivity.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Titration of PHA+PMA stimulated EL4 cell culture supernatant within the working range of the assay. EL4 cells were cultured in the presence of 1.5% phytohemagglutinin (PHA) and 10 ng/mL phorbol 12-myristate 13-acetate (PMA) for 48 hours. The cell culture supernatant was collected and measured in 2-fold dilution series. Background-subtracted data values (mean +/- SD, n = 2) are graphed.

  • EL4 cells were cultured in the absence or presence of 1.5% PHA and 10 ng/mL PMA for 48 hours. The cell culture supernatants were collected and their dilutions (as indicated) were measured with this kit. Interpolated concentrations of IL-17a adjusted for sample dilution are graphed in ng of IL-17a per mL of supernatant (mean +/- SD, n = 2). Note that IL-17a is not detectable in the untreated EL4 supernatant samples.

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参考文献

ab199081 has not yet been referenced specifically in any publications.

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