Mouse IL-17A ELISA Kit (ab199081)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.5 pg/ml
- Range: 6.25 pg/ml - 400 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
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製品名
Mouse IL-17A ELISA Kit
IL-17A キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Serum 5 4.9% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Serum 3 10.6% -
サンプルの種類
Cell culture supernatant, Serum, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
0.5 pg/ml -
検出範囲
6.25 pg/ml - 400 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum 102.5 97.7% - 105.8% Cell culture media 91.1 82.7% - 99.7% Cit plasma 103.6 102.2% - 105.8% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Mouse -
製品の概要
Mouse IL-17A ELISA Kit (ab199081) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-17A protein in cell culture supernatant, cit plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IL-17A with 0.5 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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特記事項
IL-17A is a pro-inflammatory cytokine that is secreted by a subset of activated T cells. It is a disulfide-linked homodimer with both glycosylated and non-glycosylated forms. IL-17A induces stromal cells to produce pro-inflammatory and hematopoietic cytokines, and also enhances the surface expression of ICAM1/intracellular adhesion molecule 1 in fibroblasts.
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試験プラットフォーム
Microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 1 x 96 tests 10X Mouse IL-17a Capture Antibody 1 x 600µl 1 x 600µl 10X Mouse IL-17a Detector Antibody 1 x 600µl 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 1 x 20ml Antibody Diluent CPI2 1 x 6ml 1 x 6ml Mouse IL-17a Lyophilized Recombinant Protein 2 vials 2 vials Plate Seals 1 unit 1 unit Sample Diluent 25BP 1 x 20ml 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit 1 unit Stop Solution 1 x 12ml 1 x 12ml TMB Development Solution 1 x 12ml 1 x 12ml -
研究分野
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機能
Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts. -
組織特異性
Restricted to activated memory T-cells. -
配列類似性
Belongs to the IL-17 family. -
翻訳後修飾
Found both in glycosylated and nonglycosylated forms. -
細胞内局在
Secreted. - Information by UniProt
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別名
- CTLA 8
- CTLA-8
- CTLA8
see all -
参照データベース
- Entrez Gene: 16171 Mouse
- SwissProt: Q62386 Mouse
関連製品
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Alternative Versions
画像
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Standard curve comparison between mouse IL-17A SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 9-fold increase in sensitivity.
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Background-subtracted data values (mean +/- SD) are graphed.
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Background-subtracted data values (mean +/- SD) are graphed.
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Titration of PHA+PMA stimulated EL4 cell culture supernatant within the working range of the assay. EL4 cells were cultured in the presence of 1.5% phytohemagglutinin (PHA) and 10 ng/mL phorbol 12-myristate 13-acetate (PMA) for 48 hours. The cell culture supernatant was collected and measured in 2-fold dilution series. Background-subtracted data values (mean +/- SD, n = 2) are graphed.
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EL4 cells were cultured in the absence or presence of 1.5% PHA and 10 ng/mL PMA for 48 hours. The cell culture supernatants were collected and their dilutions (as indicated) were measured with this kit. Interpolated concentrations of IL-17a adjusted for sample dilution are graphed in ng of IL-17a per mL of supernatant (mean +/- SD, n = 2). Note that IL-17a is not detectable in the untreated EL4 supernatant samples.
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To learn more about the advantages of recombinant antibodies see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab199081 は 7 報の論文で使用されています。
- Jung SH et al. Lactobacillus casei HY2782 and Pueraria lobata Root Extract Complex Ameliorates Particulate Matter-Induced Airway Inflammation in Mice by Inhibiting Th2 and Th17 Immune Responses. Prev Nutr Food Sci 27:188-197 (2022). PubMed: 35919572
- Hu M et al. MDH1 and MDH2 Promote Cell Viability of Primary AT2 Cells by Increasing Glucose Uptake. Comput Math Methods Med 2022:2023500 (2022). PubMed: 36158123
- Ghosh A et al. Chemopreventive Role of Black Tea Extract in Swiss Albino Mice Exposed to Inorganic Arsenic. Asian Pac J Cancer Prev 22:3647-3661 (2021). PubMed: 34837924
- Zhou Y et al. Puerarin improves graft bone defect through microRNA-155-3p-mediated p53/TNF-a/STAT1 signaling pathway. Int J Mol Med 46:239-251 (2020). PubMed: 32377717
- Yang X et al. IL-17a exacerbates hepatic ischemia-reperfusion injury in fatty liver by promoting neutrophil infiltration and mitochondria-driven apoptosis. J Leukoc Biol 108:1603-1613 (2020). PubMed: 32531822
- Pang L et al. Apigenin attenuates PM2.5-induced airway hyperresponsiveness and inflammation by down-regulating NF-?B in murine model of asthma. Int J Clin Exp Pathol 12:3700-3709 (2019). PubMed: 31933758
- Jia CE et al. Pendrin, an anion exchanger on lung epithelial cells, could be a novel target for lipopolysaccharide-induced acute lung injury mice. Am J Transl Res 8:981-92 (2016). PubMed: 27158384