製品の概要

  • 製品名Mouse IL-15 ELISA Kit (Interleukin-15)
    IL15 キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 0.5 ng/ml
  • 検出範囲
    0.33 ng/ml - 80 ng/ml
  • 添加回収試験

    > 100 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 137.2 115% - 150%
    Serum 135.1 125% - 149%
    Plasma 128.5 105% - 148%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Mouse
  • 製品の概要

    Abcam’s IL-15 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-15 in serum, plasma and cell culture supernatants. (Mouse IL-15 concentration is pretty low in normal serum/plasma, it may not be detected in this assay)

    This assay employs an antibody specific for mouse IL-15 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-15 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-15 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-15 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 関連性IL15 (114 amino acids) is a cytokine that regulates T and natural killer cell activation and proliferation. It has a predicted molecular mass of approximately 12.5 kDa. Human IL15 shares approximately 97% and 73% amino acid sequence identity with simian and mouse IL15, respectively. Both human and simian IL15 are active on mouse cells. IL15 was initially isolated from the simian kidney epithelial cell line CV1/EBNA. It has also been isolated from mouse and human cell sources. The cytokines IL15 and IL2 share many biological properties and stimulatory activities (T, B, and NK cells). Both IL15 and IL2 stimulate mouse CTLL2 cells. In activated peripheral blood T lymphocytes, IL2 is highly expressed but the expression of IL15 is not detectable. There is no sequence homology between IL15 and IL2, though computer modeling indicates both possess a four alpha helical bundle structure. IL15 competes for binding sites with IL2, as both IL2 and IL15 stimulate the growth of cells through the IL2 receptor. IL15 mRNA is expressed in many cell types and tissues including adherent peripheral blood mononuclear cells, fibroblasts, and epithelial cells, monocytes, placenta, and skeletal muscle.
  • 細胞内局在Isoform IL15-S48AA: Secreted. Isoform IL15-S21AA: Cytoplasm. Nucleus. Note: IL15-S21AA is not secreted, but rather is stored intracellularly, appearing in the nucleus and cytoplasmic components.
  • 別名
    • IL 15
    • Interleukin 15
    • Interleukin15
    • MGC9721
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100701 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Mouse IL-15 ELISA Kit (Interleukin-15) 画像

  • Representative standard curve using ab100701

プロトコール

Mouse IL-15 ELISA Kit (Interleukin-15) (ab100701) 使用論文

This product has been referenced in:
  • Kumar Hira S  et al. Downregulation of endogenous STAT3 augments tumoricidal activity of interleukin 15 activated dendritic cell against lymphoma and leukemia via TRAIL. Exp Cell Res 327:192-208 (2014). Sandwich ELISA ; Mouse . Read more (PubMed: 25139620) »

See 1 Publication for this product

Product Wall

Thank you for cotnacting us. The kit should contain 2 vials of the detection antibody (Item F), which is more than enough for your assay. Please let me know if you still have concerns with this kit!

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"