製品の概要

  • 製品名Mouse IL-13 ELISA Kit (Interleukin-13)
    IL13 キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 6 pg/ml
  • 検出範囲
    8.23 pg/ml - 2000 pg/ml
  • 添加回収試験

    94 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 95.37 84% - 104%
    Serum 94.42 83% - 103%
    Plasma 93.56 82% - 102%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Mouse
  • 製品の概要

    Abcam’s IL-13 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-13 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse IL-13 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-13 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-13 antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-13 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Cytokine (PubMed:8096327, PubMed:8097324). Inhibits inflammatory cytokine production (PubMed:8096327). Synergizes with IL2 in regulating interferon-gamma synthesis (PubMed:8096327). May be critical in regulating inflammatory and immune responses (PubMed:8096327, PubMed:8097324). Positively regulates IL31RA expression in macrophages.
  • 関連疾患Allergic rhinitis
  • 配列類似性Belongs to the IL-4/IL-13 family.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • Allergic rhinitis
    • ALRH
    • BHR1
    • Bronchial hyperresponsiveness 1 (bronchial asthma)
    • IL 13
    • IL-13
    • Il13
    • IL13_HUMAN
    • Interleukin 13
    • Interleukin-13
    • MGC116786
    • MGC116788
    • MGC116789
    • NC30
    • P600
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100700 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Mouse IL-13 ELISA Kit (Interleukin-13) 画像

  • Representative standard curve using ab100700

  • Representative standard curve using ab100700

プロトコール

Mouse IL-13 ELISA Kit (Interleukin-13) (ab100700) 使用論文

ab100700 has not yet been referenced specifically in any publications.

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