Mouse IL-12 p70 ELISA Kit (Interleukin-12 p70) (ab119531)

製品の概要

  • 製品名Mouse IL-12 p70 ELISA Kit (Interleukin-12 p70)
    IL12 p70 キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 7 8.3%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 7 11%
  • テスト
    1 x 96 test
  • サンプルの種類
    Cell culture supernatant, Serum
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    10 pg/ml
  • 検出範囲
    15.6 pg/ml - 1000 pg/ml
  • 添加回収試験

    106 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Serum 106 95% - 118%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Mouse
  • 製品の概要

    IL-20 p70 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IL-20 p70 in serum and cell culture supernatants.


    IL-12 p70 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with a Biotin-conjugated anti-mouse IL-12 p70 detection antibody and the microplate is then incubated at room temperature. Following washing with wash buffer a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and then unbound conjugates are washed away using wash buffer. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-12 p70 captured on the plate.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

法規制情報

製品の特性

  • 機能Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC.
  • 配列類似性Belongs to the IL-6 superfamily.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • CLMF
    • CLMF p35
    • Cytotoxic lymphocyte maturation factor 1
    • Cytotoxic lymphocyte maturation factor 1 p35
    • Cytotoxic lymphocyte maturation factor 35 kDa subunit
    • IL-12 subunit p35
    • IL-12A
    • IL12A
    • IL12A_HUMAN
    • IL35 subunit
    • Interleukin 12 alpha chain
    • Interleukin 12 p35
    • Interleukin 12 p70
    • Interleukin-12 subunit alpha
    • Natural killer cell stimulatory factor 1
    • Natural killer cell stimulatory factor 1 35 kD subunit
    • NF cell stimulatory factor chain 1
    • NFSK
    • NK cell stimulatory factor chain 1
    • NKSF1
    • P35
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab119531 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Mouse IL-12 p70 ELISA Kit (Interleukin-12 p70) 画像

  • Representative Standard Curve using ab119531.

プロトコール

Mouse IL-12 p70 ELISA Kit (Interleukin-12 p70) (ab119531) 使用論文

ab119531 has not yet been referenced specifically in any publications.

Product Wall

GOOD

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Sample: Mouse Cell (Homogenised lung tissue)
Specification: Homogenised lung tissue
Type of Kit Used: ELISA
Positive control: Virally infected lung tissue.
Did you use the Abcam Kit protocol?: Yes
Additional data
Additional Notes: Overall, a good kit.
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投稿 Jan 25 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"