Mouse IL-12 p40/p70 ELISA Kit (Interleukin-12 p40/p70) (ab100699)

製品の概要

  • 製品名Mouse IL-12 p40/p70 ELISA Kit (Interleukin-12 p40/p70)
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 5 pg/ml
  • 検出範囲
    2.74 pg/ml - 2000 pg/ml
  • 添加回収試験

    94 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 95.25 84% - 103%
    Serum 93.29 82% - 102%
    Plasma 94.52 83% - 103%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Mouse
  • 製品の概要

    Abcam’s IL-12 p40/p70 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IL-12 p40/p70 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Mouse IL-12 p40/p70 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-12 p40/p70 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-IL-12 p40/p70 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

法規制情報

製品の特性

  • 関連性IL12 is a heterodimeric cytokine comprised of p35 and p40 subunits. IL12 plays a central role in mediating cell mediated immunity, promoting differentiation of CD4+ T cells to the Th1 subset and of CD8+ T cells into mature cytotoxic T lymphocytes (CTLs). IL12 is a potent stimulator of NK cells. IL12 therapy has been suggested as a method of enhancing cytocidal anti tumour immune responses.
  • 別名
    • CLMF p35
    • CLMF p40
    • CLMF1
    • CLMF2
    • Cytotoxic lymphocyte maturation factor 1, p35
    • Cytotoxic lymphocyte maturation factor 35 kDa subunit
    • Cytotoxic lymphocyte maturation factor 40 kDa subunit
    • Il 12b
    • Il 12p40
    • IL12 subunit p35
    • IL12 subunit p40
    • IL12A
    • IL12B
    • Il12p40, p40
    • Interleukin 12 alpha chain
    • Interleukin 12 subunit alpha
    • Interleukin 12 subunit beta
    • Interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
    • Interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
    • MGC151228
    • MGC151232
    • Natural killer cell stimulatory factor 1, 35 kD subunit
    • Natural killer cell stimulatory factor, 40 kD subunit
    • NKSF
    • NKSF2 antibody
    • OTTMUSP00000005619
    • P35
    • RP23-388G23.1
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Mouse IL-12 p40/p70 ELISA Kit (Interleukin-12 p40/p70) 画像

  • Representative standard curve using ab100699

  • Representative standard curve using ab100699

プロトコール

Mouse IL-12 p40/p70 ELISA Kit (Interleukin-12 p40/p70) (ab100699) 使用論文

ab100699 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"