製品の概要

  • 製品名Mouse IL-10 ELISA Kit (Interleukin-10)
    IL10 キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    A 8 556.8pg/ml 11.6 2%
    B 8 137.4pg/ml 6.8 4.9%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    A 8 548.2pg/ml 12.1 2.2%
    B 8 133.6pg/ml 5.4 4%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 20 pg/ml
  • 検出範囲
    31.25 pg/ml - 1000 pg/ml
  • 全工程の試験時間
    3h 45m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Mouse
  • 製品の概要

    Abcam’s IL-10 mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-10 in mouse serum, buffered solutions and cell culture medium.

    A monoclonal antibody specific for IL-10 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-10 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-10 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-10 present in the samples.

    This kit will recognize both endogenous and recombinant mouse IL-10.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

法規制情報

製品の特性

  • 機能Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
  • 組織特異性Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
  • 配列類似性Belongs to the IL-10 family.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • CSIF
    • Cytokine synthesis inhibitory factor
    • GVHDS
    • IL 10
    • IL-10
    • IL10
    • IL10_HUMAN
    • IL10A
    • Interleukin 10
    • Interleukin-10
    • Interleukin10
    • MGC126450
    • MGC126451
    • T cell growth inhibitory factor
    • TGIF
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab46103 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Mouse IL-10 ELISA Kit (Interleukin-10) 画像

  • Representative Standard Curve using ab46103

プロトコール

Mouse IL-10 ELISA Kit (Interleukin-10) (ab46103) 使用論文

This product has been referenced in:
  • Savvatis K  et al. Mesenchymal stromal cells but not cardiac fibroblasts exert beneficial systemic immunomodulatory effects in experimental myocarditis. PLoS One 7:e41047 (2012). Mouse . Read more (PubMed: 22815907) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry. The antibodies used in the Murine IL10 ELISA kit are a goat polyclonal as capture antibody, and a rat monoclonal [B-C40] for detection. I hope this was the information you were looking for.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"