Mouse IL-1 beta ELISA kit (Interleukin-1 beta) (ab108866)


  • 製品名Mouse IL-1 beta ELISA kit (Interleukin-1 beta)
    IL1 beta キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    サンプル N 平均値 SD CV%
    Overall 4.3%
    サンプル N 平均値 SD CV%
    Overall 7.5%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    = 0.016 ng/ml
  • 検出範囲
    0.016 ng/ml - 1 ng/ml
  • 添加回収試験

    98 %

  • 全工程の試験時間
    5h 00m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Mouse
  • 製品の概要

    Abcam’s IL-1 beta (Interleukin-1 beta) mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of mouse IL-1 beta concentrations in plasma, serum and cell culture supernatants.

    An IL-1 beta specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an IL-1 beta specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-1 beta captured in plate.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate


  • 機能Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
  • 配列類似性Belongs to the IL-1 family.
  • ドメインThe similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
  • 細胞内局在Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
  • Information by UniProt
  • 別名
    • Catabolin
    • H1
    • Hematopoietin 1
    • IFN beta inducing factor
    • IL 1
    • IL 1 beta
    • IL 1B
    • IL-1 beta
    • IL1
    • IL1 BETA
    • IL1B
    • IL1B_HUMAN
    • IL1F2
    • Interleukin 1 beta
    • Interleukin 1 beta precursor
    • Interleukin-1 beta
    • LAF
    • OAF
    • Osteoclast activating factor
    • Preinterleukin beta
    • Pro interleukin 1 beta
    see all
  • 参照データベース


Our Abpromise guarantee covers the use of ab108866 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent dilution.

Mouse IL-1 beta ELISA kit (Interleukin-1 beta) 画像

  • Representative Standard Curve using ab108866


Mouse IL-1 beta ELISA kit (Interleukin-1 beta) (ab108866) 使用論文

ab108866 has not yet been referenced specifically in any publications.

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Gracias por contactarnos.

He querido comprobar la posibilidad de presentar reactividad cruzada entre ratón y humano en los kits contra las citoquinas que mencionas.

Tenemos varios kits contra dichas citoquinas espec&iacu...

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Thank you for your reply.

Because we carry over 100,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromi...

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Thank you for contacting us. Please be advised that we have not done any test with p-NPP substrate so we will not be able to provide any data regarding this. We have only tested this Kit using the reagent mentioned on the KIT datasheet. Regardin...

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Thank you very much for contacting us. The standard we have provided in the KIT is 2 ng/ml; which is lyophilized. We recommend adding 2 ml of Diluent Concentrate (10X) in the IL1 beta standard vial which will give final conc. of IL1 beta 1ng/ml. ...

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