Mouse IgG2a [MOPC-173] Isotype Control- ChIP Grade (ab18413)

製品の概要

  • 製品名
    Mouse IgG2a [MOPC-173] Isotype Control- ChIP Grade
    Mouse アイソタイプ・コントロール 製品一覧
  • 特異性
    This Balb/c myeloma derived clone has unknown specificity and was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed rat and human tissues.
  • アプリケーション
    適用あり: IHC-P, IHC-Fr, Flow Cyt, IP, WB, ChIP/Chip, ChIPmore details

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab18413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
ChIP/Chip Use at an assay dependent concentration. PubMed: 18636108
ChIP Use at an assay dependent concentration. PubMed: 18636108

画像

  • ChIP of ΔNp73 binding with SBE. The relative amount of ΔNp73 associated DNA as pulled down with an antibody directed against HA, is represented as a fold enrichment compared to pull-down with IgG (background, ab18413). Gene enrichment was quantified by qPCR using primers specific for the promoter regions of A) PAI-1, B) Col1a1 and C) p21WAF within the SBEs. Primers specific for PTEN (D) were used as a positive control. Pulldown antibody is shown on the x-axis, with y-axis showing fold enrichment ± SEM. * represents p-value>0.05.

参考文献

This product has been referenced in:
  • Ebata KT  et al. Vitamin C induces specific demethylation of H3K9me2 in mouse embryonic stem cells via Kdm3a/b. Epigenetics Chromatin 10:36 (2017). Read more (PubMed: 28706564) »
  • Hirosawa M  et al. Novel O-GlcNAcylation on Ser(40) of canonical H2A isoforms specific to viviparity. Sci Rep 6:31785 (2016). IHC-Fr, ICC/IF . Read more (PubMed: 27615797) »

See all 18 Publications for this product

レビューと Q&A

Thank you for your reply.

They are the same antibody (same clone), just that ab18449 has been screened for low endotoxin levels for use with cell culture.

I hope this information helps. Please contact us with any other questions.

Thank you for contacting us.

The anti-IL6 antibody ab9324 is a mouse monoclonal IgG2a. Therefore, you will need to use a mouse monoclonal IgG2a isotype control.

Therefore, you should use the mouse control IgG2a ab18413 and NOT the ...

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Thank you for contacting us.

I can confirm that ab18413 is isotype IgG2a, therefore is only suitable for one of the primary antibodies the customer is using. I have asked our products team to update the datasheet.

Apologies for an...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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