Mouse IgG2a [B12/8] - Isotype Control (ab91361)

製品の概要

  • 製品名
  • 特異性
    ab91361 was produced against a synthetic hapten, which is normally not present in Humans or animals. Therefore, this Mouse IgG2a does not react with any antigen of Human or animal cells.
  • アプリケーション
    適用あり: Flow Cytmore details
  • 免疫原

    Vegetal protein

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • バッファー
    Preservative: 0.1% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.2
  • Concentration information loading...
  • 精製度
    Protein G purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    B12/8
  • アイソタイプ
    IgG2a
  • 軽鎖の種類
    kappa
  • 研究分野
  • 別名
    • Mouse Isotype Control

アプリケーション

Our Abpromise guarantee covers the use of ab91361 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1µg for 106 cells. ab91361 is a negative isotype control

Mouse IgG2a [B12/8] - Isotype Control 画像

  • Flow cytometry analysis of normal whole blood sample labeling IgG2a with ab91361 at 1μg. 

  • Overlay histogram showing A549 cells stained with ab60720 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab60720, 0.5µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A549 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.

プロトコール

Mouse IgG2a [B12/8] - Isotype Control (ab91361) 使用論文

This product has been referenced in:
  • Abo Al Hayja M  et al. Reduced expression of peroxisome proliferator-activated receptor alpha in BAL and blood T cells of non-löfgren's sarcoidosis patients. J Inflamm (Lond) 12:28 (2015). Flow Cyt ; Human . Read more (PubMed: 25969669) »
  • Jun E  et al. Design of a multicomponent peptide-woven nanocomplex for delivery of siRNA. PLoS One 10:e0118310 (2015). Read more (PubMed: 25705892) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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