製品の概要

  • 製品名Mouse IGFBP2 ELISA Kit
    IGFBP2 キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 0.6 ng/ml
  • 検出範囲
    0.823 ng/ml - 600 ng/ml
  • 添加回収試験

    91 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 112.5 103% - 122%
    Serum 81.45 72% - 90%
    Plasma 80.22 73% - 92%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Mouse
  • 製品の概要

    Abcam’s IGFBP2 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IGFBP2 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Mouse IGFBP2 coated on a 96- well plate. Standards and samples are pipetted into the wells and IGFBP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse IGFBP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGFBP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
  • 配列類似性Contains 1 IGFBP N-terminal domain.
    Contains 1 thyroglobulin type-1 domain.
  • ドメインThe C-terminus is required for IGF-binding and growth inhibition.
  • 翻訳後修飾O-glycosylated.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • BP 2
    • BP2
    • IBP 2
    • IBP-2
    • IBP2
    • IBP2_HUMAN
    • IGF binding protein 2
    • IGF BP53
    • IGF-binding protein 2
    • IGF-BP53
    • IGFBP-2
    • IGFBP2
    • IGFBP53
    • Insulin Like Growth Factor Binding Protein 2
    • Insulin like growth factor binding protein 2 36kDa
    • Insulin like growth factor-binding protein 2 precursor
    • Insulin-like growth factor-binding protein 2
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100691 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Mouse IGFBP2 ELISA Kit 画像

プロトコール

Mouse IGFBP2 ELISA Kit (ab100691) 使用論文

This product has been referenced in:
  • Moya-Pérez A  et al. Hepatic molecular responses to Bifidobacterium pseudocatenulatum CECT 7765 in a mouse model of diet-induced obesity. Nutr Metab Cardiovasc Dis N/A:N/A (2013). Read more (PubMed: 23831006) »

See 1 Publication for this product

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Thank you for your inquiry.

I heard back from the lab that the suggested dilution for normal serum/plasma is 2 - 10 fold. They do note that levels of the target protein may vary between different specimens. Optimal dilution factors for each s...

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Sie können das Assay Diluent Bnun unter ab125050bestellen.



Leider verkaufen wir die einzelnen Kit Komponenten nicht separat, so dass ich Ihnen leider in Ihrem Fall nicht weiterhelfen kann.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"